A key step in the biosynthesis of triglycerides (fats) is the conversion of glycerol to glycerol-1- phosphate by ATP: Mg2+ Glyserol (G) + ATP glyserol-1-phosphate (G-1-P) + ADP a-glycerol kinase At a steady state in the living cell, (ATP) = 10³M and (ADP) = 104 M. The maximum (equilibrium) value of the ratio (glycerol-1-P)/(glycerol) is observed to be 770 at 25 °C and pH=7. a. Calculate K and A‚G° for the reaction. b. Using the value of AG° for the reaction ADP + P¡ →ATP +H2O, together with the answer to part (a), calculate A:G° and K for the reaction: glycerol + phosphate> glycerol-1-phosphate+ H2O_

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A key step in the biosynthesis of triglycerides (fats) is the conversion of glycerol to glycerol-1-
phosphate by ATP:
Mg2+
Glyserol (G) +
ATP
glyserol-1-phosphate (G-1-P) + ADP
a-glycerol kinase
At a steady state in the living cell, (ATP) = 10-³M and (ADP)
104 M. The maximum
(equilibrium) value of the ratio (glycerol-1-P)/(glycerol) is observed to be 770 at 25 °C
and pH=7.
a. Calculate K and A,G° for the reaction.
b. Using the value of AG° for the reaction ADP + Pi →ATP +H2O, together with the answer to
part (a), calculate A:G° and K for the reaction: glycerol+ phosphate> glycerol-1-phosphate+ H2O
Transcribed Image Text:A key step in the biosynthesis of triglycerides (fats) is the conversion of glycerol to glycerol-1- phosphate by ATP: Mg2+ Glyserol (G) + ATP glyserol-1-phosphate (G-1-P) + ADP a-glycerol kinase At a steady state in the living cell, (ATP) = 10-³M and (ADP) 104 M. The maximum (equilibrium) value of the ratio (glycerol-1-P)/(glycerol) is observed to be 770 at 25 °C and pH=7. a. Calculate K and A,G° for the reaction. b. Using the value of AG° for the reaction ADP + Pi →ATP +H2O, together with the answer to part (a), calculate A:G° and K for the reaction: glycerol+ phosphate> glycerol-1-phosphate+ H2O
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