A fermentation process was carried out with a yeast in a chemically defined medium containing glucose (C6H12O6) as a carbon source aiming at the production of ethanol (C2H6O). For the evaluation of the process, samples were taken at different times of cultivation and the experimental results are shown in the Table below. Based on the experimental results, one can: a) Calculate the overall substrate-to-cell conversion factor
A fermentation process was carried out with a yeast in a chemically defined medium containing glucose (C6H12O6) as a carbon source aiming at the production of ethanol (C2H6O). For the evaluation of the process, samples were taken at different times of cultivation and the experimental results are shown in the Table below. Based on the experimental results, one can:
a) Calculate the overall substrate-to-cell conversion factor
b) Calculate the overall substrate to product conversion factor
c) Calculate the volumetric productivity of ethanol
d) Determine the theoretical maximum conversion of glucose to ethanol
e) Determine the efficiency of the process
f) Based on the MONOD equation, calculate the maximum specific growth rate and the value of the saturation constant
g) Determine the culture generation time
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