a) describe the significance of the TCA cycle as the common catabolic pathway and explain why blocking of this cycle leads to the lethal effect of FA; b) indicate the reaction where the FA blocks the TCA cycle which is inhibitcd by FA; c) explain why concentration of citrate is increased. the enzyme

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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5. To control the damage that rats may cause in the buildings, fluoracetate (FA)
is used. After entering a cell FA is converted into very toxic fluoroacetyl-CoA.
The effect of FA was studied in the experiments on intact isolated rat hearts.
It was found after perfusion with FA the rat hearts the concentrations of the
TCA cycle metabolites were decreased. Only the level of citrate was significantly
higher than in controls.
a) describe the significance of the TCA cycle as the common catabolic
pathway and explain why blocking of this cycle leads to the lethal effect of FA;
b) indicate the reaction where the FA blocks the TCA cycle and the enzyme
which is inhibited by FA;
c) explain why concentration of citrate is increased.
Transcribed Image Text:5. To control the damage that rats may cause in the buildings, fluoracetate (FA) is used. After entering a cell FA is converted into very toxic fluoroacetyl-CoA. The effect of FA was studied in the experiments on intact isolated rat hearts. It was found after perfusion with FA the rat hearts the concentrations of the TCA cycle metabolites were decreased. Only the level of citrate was significantly higher than in controls. a) describe the significance of the TCA cycle as the common catabolic pathway and explain why blocking of this cycle leads to the lethal effect of FA; b) indicate the reaction where the FA blocks the TCA cycle and the enzyme which is inhibited by FA; c) explain why concentration of citrate is increased.
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