A calibration graph was established with a slope of 0.36 and an intercept of 0.081. The absorbance of an unknown was measured as 0.414. What is the concentration of this unknown?

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A calibration graph was established with a slope of 0.36 and an intercept of 0.081. The absorbance of an unknown was measured as 0.414. What is the concentration of this unknown?
 
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Introduction

Absorbance of a  substance is the fraction of the intensity of radiation it absorbed.

According to Beer-Lamberts law, the absorbance of a solution is directly proportional to the intensity of incident radiation and the concentration of the solution.

A plot of absorbance versus concentration  gives a straight line.

 

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