A biologists is studying the internal ultrastructure of cells of unknown source. The biologist can achieve the finest resolution by using a А. compound light microscope В. scanning electron microscope С. transmission electronic microscope D. phase-contrast light microscope
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![A biologists is studying the internal ultrastructure of cells of unknown source.
The biologist can achieve the finest resolution by using a
А.
compound light microscope
В.
scanning electron microscope
С.
transmission electronic microscope
D.
phase-contrast light microscope
Based on your answer in the previous item, what is the drawback of using this type of microscope?
А.
provides higher contrast
cannot view dynamic processes of the cell
B.
provides for higher resolving power
С.
provides for higher magnification
D.
What support can give to substantiate your answer in the previous item?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0f5f2477-ce13-4571-ac2c-b2827179e629%2F258c8f4e-2724-4ca6-b315-0cc17bbf1417%2F279a9n_processed.png&w=3840&q=75)
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- The best definition for resolution is: O The ability of an organelle to pick up stain The thickness of a specimen to be examined by microscopy The observed size divided by the actual size O The magnification of a microscope The distance between two distinguishable objectsThe 3 components that make a microscope useful in the realm of cell biology are contrast, resolution, and magnification True O FalseStaining of cells is often performed to enhance images acquired by brightfield microscopy. Phasecontrast microscopy does not require cell staining. Why is this advantageous?
- A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 10 mm) in diameter, and wants to viewa magnified image of a cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? 10X O 40X O 100X 0 4XIn order to visualize the fine structure of viruses and cytoskeletal filaments at 10-25 nanometers in diameter the type of microscopy that would be most effective is O standard light microscopy O phase-contrast light microscopy transmission electron microscopy O darkfield light microscopy O differential-interference microscopyWhich of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organelles
- A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 103 mm) in diameter, and wants to view a magnified image of a %3D cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? O 10X O 40X O 100X O 4X Question 7 2 ptsWhich of the following is never useful for observing living cells? O phase-contrast microscope scanning electron microscope O darkfield microscope O brightficld microscope confocal microscopeThe series of photos above shows you plant cells when looked at with a light microscope. The first image is taken with a 4x objective lens. We always look through an ocular lens (eyepiece of a microscope) which in this case has a 10x magnification. This means tha the total magnification for the first image is 40x (meaning 40 times as big as you could see with the naked eye). The second image is using the 10x lens, with a total magnification of 100x. The third image is taken using a 40x objective, with a total magnification of 400x! What does each box in the microscope image represent? A. each box is an organelle B. each box is an animal cell C. each box is a plant cell D. each box is a nucleus
- 3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?What description best describes the compound light microscope? Uses a beam of electrons is transmitted through a specimen to form an image. Has more than one lens and its own light source. In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. An optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.List and describe 3 factors that affect the resolution of a compound microscope What reasons could there be for failing to obtain isolated/single colonies on a streak plate? Your answer should be specific to this technique (so should NOT refer to general aseptic technique or good laboratory practice). Give TWOreasons