A biochemist needs to preserve anenzy.me 250 mL 0.50 M acetate buffer at in pH 5.5. (Ka of acetic acid ? 1.8 X 10 -5). How _would the biochemist_pre paré the puffer?- What is the pKa. of the acetic acid ?
A biochemist needs to preserve anenzy.me 250 mL 0.50 M acetate buffer at in pH 5.5. (Ka of acetic acid ? 1.8 X 10 -5). How _would the biochemist_pre paré the puffer?- What is the pKa. of the acetic acid ?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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![A biochemist_needs topreserve anenzyme
in a 250 mL D:50 M acetate buffer at
pH 5.5. (Ka of acetic accid =1.8 X 10 -5).
How would the biochemist pre pare the puffer?
What is the pxa. of the achtic acid ?
a. -4.74
b. 1.8
d. 4.74
A bio chemist needs to preserve an enzyme io
250 mL
Ka of acetic acid
would the biochemist prepare the Bufferi
0-50 M acet ate buffer at pH 5.S.
1.8 X 10-5). tHow
=
a calculate the amount otacetic acit and
todium atetate nteded-using thot pit-scate
equantion
b.Determine first the ratio dum-atetate t
acette a cid ieeded-by estimation.
ċ. Use Henderson -Hasselbalch equation to
calculate the needed ratio ofujugate pasE
and weak Acit.
t Noñe of the given choices
CS
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Transcribed Image Text:A biochemist_needs topreserve anenzyme
in a 250 mL D:50 M acetate buffer at
pH 5.5. (Ka of acetic accid =1.8 X 10 -5).
How would the biochemist pre pare the puffer?
What is the pxa. of the achtic acid ?
a. -4.74
b. 1.8
d. 4.74
A bio chemist needs to preserve an enzyme io
250 mL
Ka of acetic acid
would the biochemist prepare the Bufferi
0-50 M acet ate buffer at pH 5.S.
1.8 X 10-5). tHow
=
a calculate the amount otacetic acit and
todium atetate nteded-using thot pit-scate
equantion
b.Determine first the ratio dum-atetate t
acette a cid ieeded-by estimation.
ċ. Use Henderson -Hasselbalch equation to
calculate the needed ratio ofujugate pasE
and weak Acit.
t Noñe of the given choices
CS
Scanned with CamScanner
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