Homework Help is Here – Start Your Trial Now!
Science
Chemistry
A biochemist is purifying and characterizing an acid phosphatase from horse gram seeds. He reads the methods to be used, and then starts collecting all the materials and solutions. He will need a 0.2 M acetate buffer at pH 5.0 for multiple methods. Thus, he decides to prepare 500 mL of this buffer. He knows that this buffer is a good choice, since it is a mixture that contains both a weak acid (acetic acid) and its salt form (sodium acetate). He takes sodium acetate (MW = 82.034 g/mol), 1 M acetic acid solution, and water to prepare the solution. Describe how you would make the buffer. Choose one. show work. don't give Ai generated solution Dissolve 10.50 g of sodium acetate in minimal amounts of water. Add the 72 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 7.87 g of sodium acetate in minimal amounts of water. Add the 54 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 5.25 g of sodium acetate in minimal amounts of water. Add the 36 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 2.62 g of sodium acetate in minimal amounts of water. Add the 18 mL of 1 M acetic acid. Then, dilute to 500 mL.

A biochemist is purifying and characterizing an acid phosphatase from horse gram seeds. He reads the methods to be used, and then starts collecting all the materials and solutions. He will need a 0.2 M acetate buffer at pH 5.0 for multiple methods. Thus, he decides to prepare 500 mL of this buffer. He knows that this buffer is a good choice, since it is a mixture that contains both a weak acid (acetic acid) and its salt form (sodium acetate). He takes sodium acetate (MW = 82.034 g/mol), 1 M acetic acid solution, and water to prepare the solution. Describe how you would make the buffer. Choose one. show work. don't give Ai generated solution Dissolve 10.50 g of sodium acetate in minimal amounts of water. Add the 72 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 7.87 g of sodium acetate in minimal amounts of water. Add the 54 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 5.25 g of sodium acetate in minimal amounts of water. Add the 36 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 2.62 g of sodium acetate in minimal amounts of water. Add the 18 mL of 1 M acetic acid. Then, dilute to 500 mL.

Chemistry: The Molecular Science
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter15: Additional Aqueous Equilibria
Section: Chapter Questions
Problem 101QRT
See similar textbooks
icon
Related questions
Question
None
A biochemist is purifying and characterizing an acid phosphatase from horse gram seeds. He reads the methods to be used, and then starts collecting all the materials and solutions. He will need a 0.2 M acetate buffer at pH 5.0 for multiple methods. Thus, he decides to prepare 500 mL of this buffer. He knows that this buffer is a good choice, since it is a mixture that contains both a weak acid (acetic acid) and its salt form (sodium acetate). He takes sodium acetate (MW = 82.034 g/mol), 1 M acetic acid solution, and water to prepare the solution. Describe how you would make the buffer. Choose one. show work. don't give Ai generated solution Dissolve 10.50 g of sodium acetate in minimal amounts of water. Add the 72 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 7.87 g of sodium acetate in minimal amounts of water. Add the 54 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 5.25 g of sodium acetate in minimal amounts of water. Add the 36 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 2.62 g of sodium acetate in minimal amounts of water. Add the 18 mL of 1 M acetic acid. Then, dilute to 500 mL.
Transcribed Image Text:A biochemist is purifying and characterizing an acid phosphatase from horse gram seeds. He reads the methods to be used, and then starts collecting all the materials and solutions. He will need a 0.2 M acetate buffer at pH 5.0 for multiple methods. Thus, he decides to prepare 500 mL of this buffer. He knows that this buffer is a good choice, since it is a mixture that contains both a weak acid (acetic acid) and its salt form (sodium acetate). He takes sodium acetate (MW = 82.034 g/mol), 1 M acetic acid solution, and water to prepare the solution. Describe how you would make the buffer. Choose one. show work. don't give Ai generated solution Dissolve 10.50 g of sodium acetate in minimal amounts of water. Add the 72 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 7.87 g of sodium acetate in minimal amounts of water. Add the 54 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 5.25 g of sodium acetate in minimal amounts of water. Add the 36 mL of 1 M acetic acid. Then, dilute to 500 mL. Dissolve 2.62 g of sodium acetate in minimal amounts of water. Add the 18 mL of 1 M acetic acid. Then, dilute to 500 mL.
Expert Solution
steps

Step by step

Solved in 2 steps with 5 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry: The Molecular Science
Chemistry: The Molecular Science
Chemistry
ISBN:
9781285199047
Author:
John W. Moore, Conrad L. Stanitski
Publisher:
Cengage Learning
Chemistry: Principles and Practice
Chemistry: Principles and Practice
Chemistry
ISBN:
9780534420123
Author:
Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:
Cengage Learning
World of Chemistry, 3rd edition
World of Chemistry, 3rd edition
Chemistry
ISBN:
9781133109655
Author:
Steven S. Zumdahl, Susan L. Zumdahl, Donald J. DeCoste
Publisher:
Brooks / Cole / Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry: An Atoms First Approach
Chemistry: An Atoms First Approach
Chemistry
ISBN:
9781305079243
Author:
Steven S. Zumdahl, Susan A. Zumdahl
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781133611097
Author:
Steven S. Zumdahl
Publisher:
Cengage Learning
SEE MORE TEXTBOOKS