A bacterial suspension is standardized using a 0.5 McFarland Standard. 1 mL of that bacterial suspension is aseptically transferred to a 250-mL volumetric flask and then diluted to volume with sterile water. After this is mixed, a 2.0-mL portion of the resulting suspension is taking and transferred aseptically to a 50-mL volumetric flask, where it is diluted to volume with water. The bacterial suspension is mixed and then prepared for use in product testing.

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 8.1P
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A bacterial suspension is standardized using a 0.5 McFarland Standard. 1 mL of that bacterial
suspension is aseptically transferred to a 250-mL volumetric flask and then diluted to volume with
sterile water. After this is mixed, a 2.0-mL portion of the resulting suspension is taking and
transferred aseptically to a 50-mL volumetric flask, where it is diluted to volume with water. The
bacterial suspension is mixed and then prepared for use in product testing.

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