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Q: The BamH1 enzyme comes at a concentration of 100,000 U/ml. You are asked to digest 20 ug of DNA with…

A: The term "specific activity" describes the amount of an enzyme's enzymatic activity that is produced…

Q: If your DNA extraction were a concentration of 45ng DNA/ug, how would you prepare 500 ul of a 1…

A: The hereditary molecule deoxyribonucleic acid (DNA) transmits genetic information from one…

Q: Adenylate cyclase, which synthesizes cyclic AMP from ATP, requires two metal ions, and the enzyme…

A: Adenosine triphosphate (ATP) is an energy carrying molecule found in the cells of all living things.…

Q: ents the biochemical reaction that occurs durin

A: Introduction:-  DNA synthesis is the biological process by which a deoxyribonucleic acid(DNA)…

Q: Supercoiled DNA is slightly unwound compared to relaxed DNA and this enables it to assume a more…

A: DNA is supercoiled structure which is present inside the nucleus The coiling of Dna is done by the…

Q: Is there any situation in which DNA is made based on a RNA template? What is the enzyme involved?

A: ANSWER;-  The cycle in which DNA is synthesized having as a layout an RNA chain is called reverse…

Q: Explain the following statement : a) initiation of bacteriall DNA replication is an energy…

A: Bacterial DNA replication also occurs in 5ˊ-3ˊdirection like eukaryotic replication. Both bacteria…

Q: Ethanol promotes bonding between Na+ ions from the salt and charged phosphate group of the DNA due…

A: DNA extraction is a molecular process that involves extraction of DNA from biological samples. It…

Q: A linear piece of DNA that is 30 kb long is first cut with BamHI, then with HpaII, and, finally,…

A: The restriction enzyme is a protein and it recognizes a specific sequence of short nucleotides and…

Q: Which of the following equations are true for double-stranded DNA?  a) (%G+%T) =(%A+%C) b) (%G+%A)…

A: DNA stands for deoxyribonucleic acid. It is a genetic material that passes from one generation to…

Q: Each peak in a chromatogram corresponds to: A fluorescent ddNTP which has been released from the…

A: Ans-.  A fluorescent ddNTP which has been incorporated into the DNA fragment resulting in the…

Q: a) Explain the effect of the guanine:cytosine ratio on melting temperature of DNA. b) The…

A: (a) Guanine : cytosine ratio is the ratio of base pairs in the DNA. There are four bases in DNA -…

Q: The Alu 1 enzyme comes at a concentration of 5,000 U/ml. Its buffer comes 10X. The DNA is at a…

A: Given,- Alu 1 enzyme concentration: 5,000 U/ml- Alu 1 enzyme buffer concentration: 10X- DNA…

Q: Deamination of adenine results in the formation of hypoxanthine. Hypoxanthine selectively base pairs…

A: The question is asking about the potential outcome of a specific type of mutation in DNA,…

Q: The role of iodine in the Carruthers/Letsinger method of solid-phase DNA synthesis is to act as…

A: Letsinger in 1960's targeted oligonucleotide synthesis using solid phase chemistry. Solid phase…

Q: Instructions: Put the following terms into a word map to explain how they are interrelated for DNA…

A: DNA has the ability to self replicate. It undergoes semi-conservative replication. DNA Replication…

Q: a) Under normal conditions E. coli produces three DNA polymerases. State their functional…

A: E.coli bacteria are considered prokaryotes, and the prokaryotes exhibit three different types of DNA…

Q: Draw a Lineweaver-Burke plot with two lines, one representing an uninhibited enzyme and another…

A: Competitive inhibition of Lineweaver–Burk plot increases the slope of the line and alters the…

Q: If DNA synthesis occurs without error, the resulting double-stranded DNA is an exact copy of the…

A: DNA synthesis is a process in which a double-stranded DNA molecule is created from two…

Q: The complementary strands of DNA in the double helixare held together by hydrogen bonds: G ≡ C or A…

A: DNA double helix is formed by the hydrogen bonds formed between the base pairs. Adenines forms two…

Q: The estimated half-life for P-O bonds is 1.3 x 105 a (a = annum = year). Approximately 109 such…

A: DNA (Deoxyribonucleic acid) and RNA are two examples of nucleic acids (Ribonucleic acid). To…

Q: (a) (b) (c) The figure shown below is the mechanism of a DNA nuclease. The DNA molecule is in blue…

A: The given figure shows the mechanism of a DNA nuclease. Nucleases breaks the phosphodiester bonds of…

Q: Show the expected labeled cleavage products if the following DNA segment were subjected to each of…

A: D.N.A. Cleavage ---  A cleavage of single - stranded DNA oligonucleotides in the presence of ionic…

Q: It was once thought that the DNA polymerase machinery moves along DNA in a manner analogous to a…

A: DNA polymerase is defined as the family of enzymes responsible for catalyzing the synthesis of new…

Q: DNA polymerase I, DNA ligase, and topoisomerase I catalyze the formation of phosphodiester bonds.…

A: The DNA replication is a semi-conservative mechanism in which the new complementary strand is…

Q: During high stress environments, it has been found that some bacteria activate a genetic mechanism…

A: The given enzymes are used in the replication process. It is a process wherein the genetic material,…

Q: The enzyme ornithine decarboxylase generates a product that has been proposed to play a role in…

A: Ornithine decarboxylase is an important enzyme for cell proliferation under normal growth and tumor…

Q: Some antibiotic drugs fight infection by interfering with DNA replication, transcription, or…

A: The central dogma of molecular biology explains how the instructions within the DNA of an organism…

Q: The following statements are correct EXCEPT: A) Information in the DNA is transcribed int mRNA and…

A: DNA is a genetic material which is safely stored inside the nucleus of the cell . It is ladder like…

Q: Match the following functions to each of the enzymes listed below. Some Functions of Enzymes Build…

A: DNA is the nucleic acids present in the organisms. DNA is the deoxy-ribose nucleic acid in which…

Q: Of the four DNA bases, why is guanine most likely to be modified after exposure to polycyclic…

A: DNA is made up of four bases-adenine, cytosine, guanine, and thymine. The pKa of adenine and…

Q: Considering prokaryotes, what term adds nucleotides in the 5' to 3' direction during DNA…

A: Ans- During DNA Replication process synthesis of new DNA strands takes place, using parental strands…