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A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as shown below) to 100 mL with water.

A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as shown below) to 100 mL with water.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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4. A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as shown below) to 100 mL with water. Volume used from Buffer Final volume, mL original solution, mL 1 5 100 2 10 100 20 100 a. Do they have the same pH? Check the box corresponding your answer. O Yes O No HY 47.1 Basic Biochemistry, Laboratory b. Which is true about their absolute concentrations? Check the box corresponding your answer. [Buffer 1] > [Buffer 2] > [Buffer 3] [Buffer 3] > [Buffer 2] > [Buffer 1] [Buffer 3] = [Buffer 2] = [Buffer 1] [Buffer 3] = [Buffer 2] > [Buffer 1] [Buffer 3] > [Buffer 2] = [Buffer 1] c. Which is true about their concentration ratios? Check the box corresponding your answer. [Buffer 1] > [Buffer 2] > [Buffer 3] [Buffer 3] > [Buffer 2] > [Buffer 1] [Buffer 3] = [Buffer 2] = [Buffer 1] [Buffer 3] = [Buffer 2] > [Buffer 1] [Buffer 3] > [Buffer 2] = [Buffer 1] %3! %3D d. Which of the following is true about the ability of the three buffers to resist pH change when 1 mL of HCI is added to each buffer? Check the box corresponding your answer. Ability to resist pH change: Buffer 1< Buffer 2 < Buffer 3 Ability to resist pH change: Buffer 1 > Buffer 2 > Buffer 3 Ability to resist pH change: Buffer 1 = Buffer 2 = Buffer 3 Ability to resist pH change: Buffer 1 < Buffer 2 = Buffer 3 Ability to resist pH change: Buffer 1 = Buffer 2 < Buffer 3 e. Which solution has the highest buffer capacity? Check the box corresponding your answer. Buffer 1 Buffer 2 Buffer 3 Their buffer capacities are equal
Transcribed Image Text:4. A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as shown below) to 100 mL with water. Volume used from Buffer Final volume, mL original solution, mL 1 5 100 2 10 100 20 100 a. Do they have the same pH? Check the box corresponding your answer. O Yes O No HY 47.1 Basic Biochemistry, Laboratory b. Which is true about their absolute concentrations? Check the box corresponding your answer. [Buffer 1] > [Buffer 2] > [Buffer 3] [Buffer 3] > [Buffer 2] > [Buffer 1] [Buffer 3] = [Buffer 2] = [Buffer 1] [Buffer 3] = [Buffer 2] > [Buffer 1] [Buffer 3] > [Buffer 2] = [Buffer 1] c. Which is true about their concentration ratios? Check the box corresponding your answer. [Buffer 1] > [Buffer 2] > [Buffer 3] [Buffer 3] > [Buffer 2] > [Buffer 1] [Buffer 3] = [Buffer 2] = [Buffer 1] [Buffer 3] = [Buffer 2] > [Buffer 1] [Buffer 3] > [Buffer 2] = [Buffer 1] %3! %3D d. Which of the following is true about the ability of the three buffers to resist pH change when 1 mL of HCI is added to each buffer? Check the box corresponding your answer. Ability to resist pH change: Buffer 1< Buffer 2 < Buffer 3 Ability to resist pH change: Buffer 1 > Buffer 2 > Buffer 3 Ability to resist pH change: Buffer 1 = Buffer 2 = Buffer 3 Ability to resist pH change: Buffer 1 < Buffer 2 = Buffer 3 Ability to resist pH change: Buffer 1 = Buffer 2 < Buffer 3 e. Which solution has the highest buffer capacity? Check the box corresponding your answer. Buffer 1 Buffer 2 Buffer 3 Their buffer capacities are equal
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