7. Consider the nonenzymatic elementary reaction A B. When the concentration of A is 20 mM, the reaction velocity is measured as 5 uM B produced per minute. Calculate the rate constant for this reaction. 8. The hypothetical elementary reaction 2A→B+C has a rate constant of 10-6 M, s-, what is the reaction velocity when the concentration of A is 10 mM? 9. Calculate K,and Vmax from the following data (use the Lineweaver Burk Plot) 0.1 0.2 0.4 0.8 1.6 0.34 0.53 0.74 0.91 1.04 10. Enzyme A catalyzes the reaction S-P and has a K" of 50 uM and a Vmax of 100 nMs". Enzyme B catalyzes the reaction S-Q and has a Km of 5 mM and a Vmax of 120 nM s . When 100 μΜ of S is added to a mixture containing equivalent amounts of enzymes A and B, after 1 minute which reaction product will be more abundant: P or Q?

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7. Consider the nonenzymatic elementary reaction A B. When the concentration of A is 20
mM, the reaction velocity is measured as 5 uM B produced per minute. Calculate the rate
constant for this reaction.
8. The hypothetical elementary reaction 2A→B+C has a rate constant of 10-6 M, s-, what is
the reaction velocity when the concentration of A is 10 mM?
9. Calculate K,and Vmax from the following data (use the Lineweaver Burk Plot)
0.1
0.2
0.4
0.8
1.6
0.34
0.53
0.74
0.91
1.04
10. Enzyme A catalyzes the reaction S-P and has a K" of 50 uM and a Vmax of 100 nMs".
Enzyme B catalyzes the reaction S-Q and has a Km of 5 mM and a Vmax of 120 nM s . When
100 μΜ of S is added to a mixture containing equivalent amounts of enzymes A and B, after 1
minute which reaction product will be more abundant: P or Q?
Transcribed Image Text:7. Consider the nonenzymatic elementary reaction A B. When the concentration of A is 20 mM, the reaction velocity is measured as 5 uM B produced per minute. Calculate the rate constant for this reaction. 8. The hypothetical elementary reaction 2A→B+C has a rate constant of 10-6 M, s-, what is the reaction velocity when the concentration of A is 10 mM? 9. Calculate K,and Vmax from the following data (use the Lineweaver Burk Plot) 0.1 0.2 0.4 0.8 1.6 0.34 0.53 0.74 0.91 1.04 10. Enzyme A catalyzes the reaction S-P and has a K" of 50 uM and a Vmax of 100 nMs". Enzyme B catalyzes the reaction S-Q and has a Km of 5 mM and a Vmax of 120 nM s . When 100 μΜ of S is added to a mixture containing equivalent amounts of enzymes A and B, after 1 minute which reaction product will be more abundant: P or Q?
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