7. Calculate the isoelectric point (pl) of the given peptide below. HO pKa = 10.46 pKa = 9.78 H3N H3C pka 2.16 pka = 10.54 -NH ΝΗ HN -NH3 -NH NH- pka = 3.90 pKa = 4.07
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- What is the isoelectric point of the following peptide molecule? Asp-His-Glu-Gly-Lys-Tyr-Leu-Phe-Arg-Ser-Cys Relevant pKa values are; 2.2, 3.8, 4.3, 6.0, 8.3, 9.8, 10.1, 10.5, 12.5 and 13.1 3.0 9.1 7.2 5.2 4.1 ONLY one correct answerCalculate the isoelectric point (pI) of the peptide structure shown. Use table 3.1 for pKa values.1) Indicate the total charge of the peptide at pH 7.0, 9.0, and 11.0 2) Determine the isoelectric point (pI) for this peptide. Show the relevant pKa’s and your calculation. 3) What is the fraction of the peptide that has the N-terminal amino group deprotonated at pH 9.
- What is the isoelectric point of the peptide LIKES? Show your work below.16. A quantitative study of the interaction of a protein with its ligand yielded the following results: Ligand concentration 0.5 1 2 3 4 5 6 9. 12 (μM) v (moles of 0.14 0.31 0.48 0.61 0.68 0.72 0.77 0.79 0.81 ligand bound per mole of protein) Plot a graph of ligand concentration versus v. Determine KD, the dissociation constant for the interaction between the protein and its ligand, from the graph.Consider the equilibrium of arginine below: NH₂ NH₂ H₂N: NH OH H₂N- pKa=2.17 NH3 NH H₂NE pKa2=9.04 NH3 03 NH₂ NH HN: pka 12.48 NH₂ I II III If the pH of arginine solution is 8.08, what would be the major form of arginine? NH₂ NH 00 IV NH₂
- K₁ = α [H+][A-] [HA] [A-] pH = pK+log [HA] 1-1 The Amino acid Arginine ionizes according to the scheme below. Calculate the isoelectric point of Arginine and the average charge on arginine when pH = 9.2. NH2 H C=N NH pka-2.17 -H' NH2 H C=N H NH PK-8.99 -H +H* H (CH2)3 H-N-C-COOH | H H +H* H (CH2)3 H-N-C-coo- H H Ans: 10.75; +0.38 NH2 NH2 H C=N C=N-H H NH PK-12.5 -H NH | +H* (CH2)3 H₂N-C-COO- | H III (CH2)3 H₂N-C COO- H IV16.B. The formula for "Lubow's Solution" is as follows3: 1 g 500 mg Progesterone Hydrocortisone 2 g Propylene glycol Ethanol (95%) ad 100 mL (a) Calculate the quantities of each ingredient required to prepare 2.5 mL of solution. (b) How many milliliters of propylene glycol (sp. gr. 1.04) are needed to prepare a liter of the formula?1. Draw the peptide chain (indicate if it is solid or hatched wedges) for the following and compute for the isoelectric point. Show solution. Glycine-(L)-Arginine-(D)-Proline-(L)-Glutamic Acid
- 4. a. Give the full names of the amino acids in this peptide. НзN — CH—С-N—CH— С-N—CH—С—О ČH3 H ČH,OH H. CH2 -ОН b. Calculate the isoelectric point (pl) of a peptide with sequence SNARE. Show your work in a table, giving the pKa values of the ionizable groups, pH, and charges. Step marks are counted. Don't need to write “protonated" or “deprotonated".A4Give the complete name of the peptide below.