6. You discover a new transmembrane ion channel and upon structural characterization you demonstrate that it is a homo-tetramer, in which each subunit possess 2 transmembrane a- perpendicular to the plane of the membrane and 20 amino acid residues are embedded in the lipid bilayer. a. Estimate the thickness of the membrane lipid bilayer. b. If the homotetramer has MW of 70 KDa, what percent of its mass would reside in the membrane-spanning portion? c. Upon conformational change one of the two helices in each subunit tilts to 45 degrees within the membrane in respect to the plane. How many amino acids in this helix are embedded in the membrane after the transition? d. What percent of the ion channel mass reside in the membrane-spanning portion after the conformational change occurs? e. Transmembrane pores and channels can also be formed by proteins that form B-barrel structures. B-barrels are constituted by ß-strands that spans the entire membrane. Assuming that these B-strands are perpendicular to the plane of the membrane, how many residues must a B-strand have to span the lipid bilayer? "

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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6. You discover a new transmembrane ion channel and upon structural characterization
you demonstrate that it is a homo-tetramer, in which each subunit possess 2 transmembrane a-
helices spanning the entire length of the membrane. Both of these helices are perfectly
perpendicular to the plane of the membrane and 20 amino acid residues are embedded in the
lipid bilayer.
a. Estimate the thickness of the membrane lipid bilayer.
b. If the homotetramer has MW of 70 KDa, what percent of its mass would reside in the
membrane-spanning portion?
c. Upon conformational change one of the two helices in each subunit tilts to 45 degrees
within the membrane in respect to the plane. How many amino acids in this helix are
embedded in the membrane after the transition?
d. What percent of the ion channel mass reside in the membrane-spanning portion after the
conformational change occurs?
e. Transmembrane pores and channels can also be formed by proteins that form B-barrel
structures. B-barrels are constituted by B-strands that spans the entire membrane. Assuming
that these B-strands are perpendicular to the plane of the membrane, how many residues
must a B-strand have to span the lipid bilayer?
Transcribed Image Text:6. You discover a new transmembrane ion channel and upon structural characterization you demonstrate that it is a homo-tetramer, in which each subunit possess 2 transmembrane a- helices spanning the entire length of the membrane. Both of these helices are perfectly perpendicular to the plane of the membrane and 20 amino acid residues are embedded in the lipid bilayer. a. Estimate the thickness of the membrane lipid bilayer. b. If the homotetramer has MW of 70 KDa, what percent of its mass would reside in the membrane-spanning portion? c. Upon conformational change one of the two helices in each subunit tilts to 45 degrees within the membrane in respect to the plane. How many amino acids in this helix are embedded in the membrane after the transition? d. What percent of the ion channel mass reside in the membrane-spanning portion after the conformational change occurs? e. Transmembrane pores and channels can also be formed by proteins that form B-barrel structures. B-barrels are constituted by B-strands that spans the entire membrane. Assuming that these B-strands are perpendicular to the plane of the membrane, how many residues must a B-strand have to span the lipid bilayer?
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