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6. Determine the Km and Vmax from the LB plot for enzyme assays in buffer 1 and buffer 2. Buffer 1 Buffer 2 Km (mm) Vmax (μM/min)

6. Determine the Km and Vmax from the LB plot for enzyme assays in buffer 1 and buffer 2. Buffer 1 Buffer 2 Km (mm) Vmax (μM/min)

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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How to calculate Km value and Vmax in buffer1 and 2
1/ Final [PNPP] (mM-1) 2.5 2 1.5 0.5 0 Km (mm) Vmax (μM/min) Lineweaver-Burk (LB) plot 1/ Final [PNPP] (mM-1) vs 1/VO (min/µM) y = 7.9966x -0.6983 Buffer 1 0.2 0.4 0.6 0.8 1/V0 (min/μM) y = 1.6343x -0.0839 Buffer 2 1 6. Determine the Km and Vmax from the LB plot for enzyme assays in buffer 1 and buffer 2. Buffer 1 Buffer 2 1.2 1.4
Transcribed Image Text:1/ Final [PNPP] (mM-1) 2.5 2 1.5 0.5 0 Km (mm) Vmax (μM/min) Lineweaver-Burk (LB) plot 1/ Final [PNPP] (mM-1) vs 1/VO (min/µM) y = 7.9966x -0.6983 Buffer 1 0.2 0.4 0.6 0.8 1/V0 (min/μM) y = 1.6343x -0.0839 Buffer 2 1 6. Determine the Km and Vmax from the LB plot for enzyme assays in buffer 1 and buffer 2. Buffer 1 Buffer 2 1.2 1.4
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To determine the value of Km and Vmax from the given Lineweaver-Burk plot for buffer-1 and buffer 2. 

 

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