CONTENTS OF REACTION CUVETTES: (Calculations in PreLab)[ONPG]Diluted ONPG0mMNone0.25mL0.5mL0.75mL1.0mL bloo1.25mL1.5mLBufferLactase0.25mL0.25mL0.25mL0.25mL0.25mL0.25mL0.25mL0.25mL0.25mL0.25mL0.25mL0.25mL0.25mL0.25mL*** HINT: To calculate [ONPG], cuvette #5 contains 1.0mL ONPG in a total of 2.0mL. (This is the same as a 1:2dilution.) Therefore we divide the concentration of ONPG by 2 to get 5mM.5mMWater1.5mL1.25mL1.0mL0.75mL0.5mL0.25mLNone***4. To perform Lactase Assay with first cuvette ("OmM"):Clean cuvette with Kimwipe.Place cuvette into Spectro Vis with stripes in front in back.GET GROUP READY FOR TIMED ASSAY. AT THE SAME TIME:* Press Record (Green Triangle button).* Add 0.25mL of Lactase to cuvette. Use pipette to quickly mix liquid.WAIT 60 SECONDS! After the assay is complete:* In Experiment menu, select "Store Latest Run" THIS STEP IS NECESSARY!* Record data on the data table below.5. Repeat Step 7 for all of your cuvettes. Record the data below.2viiboloV\:8A11201 201sall rade5. What is the final ONPG concentration (in mM) of each of the Reaction tubes?***Hint: Use the table on in your lab manual to figure this out. ***k moHatesgoutmM of ONPG in Reaction tube= 0mL of ONPG in a total volume of 2mL = 0 (10mM) = 0mM= 0.25 mL of ONPG in a total volume of 2mL = (0.25*10mM)/2 = 1.25mMmL of ONPG in a total volume of 2mL = (______ *10mM)/2 =mM== 1.0mL of ONPG in a total volume of 2mL = ½ (10mm) = 5mMMacBook Pro

The dilution formula is used to calculate the concentration of a solution after it has been diluted.…

Answered: 5. What is the final ONPG concentration…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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