5. The MLT performs patient testing against specific reagent anti-sera and obtains the following results:Anti-eAnti-D4+Anti-c3+3+Anti-C3+a) What is this patient's Rh phenotype?Anti-E0

The Rh phenotype of a patient is decided by the presence or absence of particular Rh antigens on the…

Answered: 5. The MLT performs patient testing…

Phlebotomy Essentials

6th Edition

ISBN:9781451194524

Author:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Publisher:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Chapter1: Phlebotomy: Past And Present And The Healthcare Setting

Section: Chapter Questions

Problem 1SRQ

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3. Discuss the applications of the direct and indirect antiglobulin tests.
A patient is O positive and his serum is agglutinating all screening and panel reagent cells at room temperature. His autocontrol is positive at room temperature also. The patient may have an antibody to a high incidence antigen. One possibility is that he may have a clinically insignificant autoanti-I. How could you prove that the patient has an anti-I antibody?
16. The Type A Transferase enzyme, attaches N-acetylgalactosamine (GalNAc) to H-antigen epitopes; the Type B Transferase enzyme instead transfers galactose (Gal) to the H-antigen. Individuals with Type A blood thus have at minimum one copy of the active Type A Transferase, those with Type B have at minimum one copy of the active Type B Transferase, those with Type AB have one active copy of each, those with Type O have neither. Imagine an AB individual who acquires a mutation in their Type B Transferase gene that allows it to be active with either Gal or GalNAc. Make sure to include a brief justification for your answer to each question below. a. What blood type antigens would this individual express? b. If this individual had a child with a normal O partner, what possible blood type antigens could the child express? c. If this individual had a child with a homozygous dominant B partner (BB), what possible blood type antigens could the child express?
1. Read through the introductory information and use it to fill in the table below. For each blood type, which antigens are present on the red blood cells? Which antibodies would you expect that person to have against the antigens not present on their red blood cells? Blood Type A B AB O RH+ RH- Antigens Present Antibodies present
Describe in details for each of the following: 1. SARS-CoV2 mode of transmission 2. COVID-19 diagnostic test 3. COVID-19 antibody test
5. A patient receives a donor kidney to replace diseased kidney tissue. Consider and answer the following: C) Immunosuppressive therapy is most commonly applied after tissue transplantation. Describe another method that can be used to encourage graft survival.
1. What are the general (basic) functions of antibodies relative to APS? Please include citation (s).
4. What is the rationale behind the sequence of the order of draw? Sterile tube (blood culture) a. b. Blue-top coagulation tube C. Serum tube (glass) with no additive d. Serum tube with clot activator, with or without e. Heparin tube with or without gel plasma separator f. EDTA tube g. Glycolytic inhibitor tube tubo with silica clc
1.
1. Serum levels of which antibody is indicative of recent infection?? which antibody isotypes remains primarily in serum? IgG, IgA, IgE, IgD, IgM
1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8
state the significance of heterophile antibodies in serological testing

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5. The MLT performs patient testing against specific reagent anti-sera and obtains the following results: Anti-E Anti-e Anti-D 4+ Anti-c 3+ 0 3+ Anti-C 3+ a) What is this patient's Rh phenotype?