40. If you are given only the first six data points (from [S] = 2.5 x 103 mM to 0.1 mM) in the table above, how would you accurately compute Km and Vmax? [S] (mM) Vo w/o inhib (μmol/min) Vo w/ inhib (μmol/min) 2.5 x 10-3 28 13 4 x 10-3 40 22 0.01 70 38 0.02 95 50 0.04 112 70 0.1 128 90 2 139 115 3579 139.5 125 139.5 132 140 137 10 140 139

40. If you are given only the first six data points (from [S] = 2.5 x 103 mM to 0.1 mM) in the table above, how would you accurately compute Km and Vmax? [S] (mM) Vo w/o inhib (μmol/min) Vo w/ inhib (μmol/min) 2.5 x 10-3 28 13 4 x 10-3 40 22 0.01 70 38 0.02 95 50 0.04 112 70 0.1 128 90 2 139 115 3579 139.5 125 139.5 132 140 137 10 140 139

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

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Question

40. If you are given only the first six data points (from [S] = 2.5 x 103 mM to 0.1 mM) in the table
above, how would you accurately compute Km and Vmax?
[S]
(mM)
Vo w/o inhib
(μmol/min)
Vo w/ inhib
(μmol/min)
2.5 x 10-3
28
13
4 x 10-3
40
22
0.01
70
38
0.02
95
50
0.04
112
70
0.1
128
90
2
139
115
3
139.5
125
5
139.5
132
7
140
137
10
140
139

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