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4. A) Why is high pressure pump needed in HPLC. B) Why is UPLC is with a higher efficiency than HPLC. C) What are the advantages & disadvantages of suing UPLC over HPLC?

4. A) Why is high pressure pump needed in HPLC. B) Why is UPLC is with a higher efficiency than HPLC. C) What are the advantages & disadvantages of suing UPLC over HPLC?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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4. A) Why is high pressure pump needed in HPLC. B) Why is UPLC is with a higher efficiency than HPLC. C) What are the advantages & disadvantages of suing UPLC over HPLC? 5. Explain the rational for each of the steps for flushing a C18 column. 1) flush the column initially with 5-10 column volumes of the most recent mobile phase without buffer; 2) flush with 10-20 volumes of strong solvent (90% acetonitrile); 3) Store the column in strong solvent. 4) Equilibrate with 10-2- volumes of new, desired, buffered mobile phase. 6. Label on the diagram on the below components 1 to 5 of a high-performance liquid chromatographic system. Briefly describe the function of each com nent. 3 2 4 Flow controller Waste 7. Label on the diagram on the above components 1 to 5 of a gas chromatographic system. Briefly describe the function of each component. 8. Use your own words, explain what does each of the following terms mean in analytical chemistry. a) Qualitative analysis; b) Quantitative analysis. c) Sensitivity; d) Detection limit. e) Selectivity; f) Accuracy; g) Precision; h) Robust; i) Calibration check; j) Quality control sample; k) Recovery; 1) Reagent blank; m) Field blank. 9. Use your own words, suggest with explanation two of the methods that can be used to gauge method accuracy in chemical analysis. 10. The concentration of benzene in winter rainwater is 0.3 ppb. Assuming that the density of rainwater is close to 1.00 g/mL. Express the concentration of benzene in ppm, ppt, g/L, µg/L, µg/mL, mg/L, and find the molar concentration. 11. A 85 wt% propanol in water has a density of 0.80 g/mL. Find the mass of 1.0 L of this solution, the grams of propanol per liter, and the molar concentration of propanol in this solution.
Transcribed Image Text:4. A) Why is high pressure pump needed in HPLC. B) Why is UPLC is with a higher efficiency than HPLC. C) What are the advantages & disadvantages of suing UPLC over HPLC? 5. Explain the rational for each of the steps for flushing a C18 column. 1) flush the column initially with 5-10 column volumes of the most recent mobile phase without buffer; 2) flush with 10-20 volumes of strong solvent (90% acetonitrile); 3) Store the column in strong solvent. 4) Equilibrate with 10-2- volumes of new, desired, buffered mobile phase. 6. Label on the diagram on the below components 1 to 5 of a high-performance liquid chromatographic system. Briefly describe the function of each com nent. 3 2 4 Flow controller Waste 7. Label on the diagram on the above components 1 to 5 of a gas chromatographic system. Briefly describe the function of each component. 8. Use your own words, explain what does each of the following terms mean in analytical chemistry. a) Qualitative analysis; b) Quantitative analysis. c) Sensitivity; d) Detection limit. e) Selectivity; f) Accuracy; g) Precision; h) Robust; i) Calibration check; j) Quality control sample; k) Recovery; 1) Reagent blank; m) Field blank. 9. Use your own words, suggest with explanation two of the methods that can be used to gauge method accuracy in chemical analysis. 10. The concentration of benzene in winter rainwater is 0.3 ppb. Assuming that the density of rainwater is close to 1.00 g/mL. Express the concentration of benzene in ppm, ppt, g/L, µg/L, µg/mL, mg/L, and find the molar concentration. 11. A 85 wt% propanol in water has a density of 0.80 g/mL. Find the mass of 1.0 L of this solution, the grams of propanol per liter, and the molar concentration of propanol in this solution.
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