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Q: What cause the spikes in the spectrophotometer reading? Explain

A: In electronic spectroscopy, generally the electron excites  from an initial low energy state to a…

Q: Single standard addition method was.16 applied for spectrophotometric analysis of Na+ in a 25 mL…

A: Let us suppose the amount of Na in unknown water sample be x ppm. If we are added the solution 0.500…

Q: How will mistakenly using solution 1 to calibrate the spectrophotometer, instead of the blank effect…

A: Blank solution is used to calibrate the absorbance with Transmittance(%T) and set a zero value by…

Q: Spectrophotometer device must contain the photocell. 2- The main differences in parts of colorimeter…

A: 1. A photocell is a device used to convert light energy into electrical energy.

Q: Anion and cation chromatography can be achieved by modifying?   A. Type of dye B. Mobile phase C.…

A: Chromatography is a technique for the separation of a mixture into its components.

Q: Table 1: Chromatography Results Mobile Phase: Distilled Water Solvent front distance (cm): 8 cm…

A: Retention factor (Rf factor):- It is the ratio of the distance travelled by the compound and the…

Q: 12. This detector is not used in HPLC: a) ECD b) MS c) Fluorescence d) RI

A: Mixture components are estimated using HPLC detectors. The actual separation in HPLC (High…

Q: 4. Give the function of the following parts of the spectrophotometer. b. Collimator C. Slit d.…

A: (a)Collimator-It is a device for changing the diverging light or other radiation from a point source…

Q: fill in the column thats empty by finding the standard deviation for each average absorbance, with…

A: Concentration of Dye  Average Absorbance of replicate   Standard division 1mg/L 0.269 0.731…

Q: Determination of Iron Content using Flame Atomic Absorption Spectrophotometry   QUESTIONS: Why is…

A: Flame atomic absorption spectroscopy is used to determine the presence of metals and metalloids in…

Q: How does the wavelength in chromatogram affect the signal intensity in an HPLC

A: wavelength in chromatogram affect the signal intensity in an HPLC explanation is given below.

Q: How does paper chromatography allow you to separate substances?

A: Paper chromatography allows you to separate substances has to be determined below.

Q: 2. What are mobile and Stationary phases in a chromatogram? Site their nature.

A: The solution is given below -

Q: Describe one alternative column other than a C18 for caffeine detection in terms of stationary phase

A: C18 columns are present in high performance liquid chromatography (HPLC) that are utilized as a…

Q: Question 7 What variations are tolerated by an analytical method is described by the method's Select…

A: The correct option is:

Q: What is the %composition of the Vitamin E peak in the sample? 20.01% 21.57%

A: To calculate % composition of vitamin E in the sample.

Q: HPLC VFA analysis - why do I get so different peaks for very similar samples?

A: Volatile fatty acids are formed during microbial fermentation of organic matter and are present in a…

Q: River water analysis of mercury (Hg) yields a spectrophotometric sample signal of 0.712 for a 5.00…

A: Before the addition of the spike, 5.00 mL of the river water sample gives an absorbance of 0.712.…

Q: Which Is The Most Commonly Used Detector In High Performance Liquid Chromatography And Why

A: High Performance Liquid Chromatography is abbreviated as HPLC. High performance liquid…

Q: Why do we use a makeup gas for some gas chromatography detectors?

A: The method of separation of components present in the mixture is termed as chromatography.…

Q: 5. Draw and label a box diagram for a double-beam spectrophotometer. What advantages does a double…

A: diagram :

Q: How can TLC be used to determine if a sample is pure?

A: Thin Layer Chromatography (TLC) is a separation procedure that requires a very small sample. It is…

Q: what is the difference of spectrophotometer with ATR (attenuated total reflectance) and one without…

A: Attenuated total reflectance (ATR) is a special accessory unit which can be used with Fourier…

Q: What is the purpose of reference beam in the double-beam spectrophotometer shown in Figure 20-1?…

A: As the name suggests, a double beam spectrophotometer employs two types of beams: reference beam and…

Q: Give reason for: 3- A sample must be bombarded with electrons in mass spectrophotometry. 4- X-axis…

A: In mass spectrophotometry, by an electron bombardment, the sample is converted to fast moving…

Q: Explain why the dye spots are elongated and not round on your chromatogram.

A: The term chromatography or the paper chromatography refers to a technique which is used for the…

Q: Retention time is the time taken for the mobile phase to reach the detector.   True False

A: Answer - The above statement is false  Explanation - Retention time - Retention time is the sum of…

Q: what chromatography seperation how is tlc part of it

A:

Q: how does TLC determine if a sample is pure?

A: checking for purity of a sample,  the known sample which is pure(having only one spot) and has the…

Q: The ppm concentration of Pb2+ in a blood sample were measured with Spectrophotometry. 5.00 mL of a…

A: To find out the concentration of Pb2+ in a blood sample given that the 5.00 mL sample of a blood…

Q: How is the signal intensity seen in the chromatogram affected by the wavelength used for analysis?

A: Chromatography is a group of  methods for separating very small quantities of complex mixtures, with…