4. a) Identify the structure shown below and label the two parts in the interior of the structure. Microvilli lymph vessel b) Which part in the diagram above is responsible for absorbing amino acids and glucose?
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- While endocytosis and exocytosis refer to the direction in which a vesicle moves into and out of a cell respectively, it is important to think about what is being moved with and within the vesicles. To demonstrate how this process is utilized in human cells we will focus on the human body's insulin response. The purpose of the insulin response is to create a pathway for glucose entry into cells by releasing glucose transporters into the membrane. Cell Type #1- insulin producers: In humans, pancreatic B-cells create steroid insulin that can be packaged into vesicles starting at the ER. These vesicles are prepared for exocytosis so that the insulin can be released from the pancreatic B - cells and get into the bloodstream when signaled to do so. When glucose is present in the blood stream these cells will release the pre-packed insulin into the blood stream. Cell Type #2- insulin responders: Other cells do not produce insulin but instead respond when they have received the insulin signal…F B A Identify the diagram. D 16 E 00 с C a) Where does this double layer occur? b) What type of molecules can pass through the double layer? c) What type of molecules cannot pass through the double layer? d) The molecules that can not pass through the double layer, how do they pass through?Deduce the amino acid sequence of a polypeptide from the following:1. Acid hydrolysis gives Ala2, Arg, Lys2, Met, Phe, Ser22. Carboxypeptidase gives Ala3. Trypsin digestion gives 4 peptides a) Ala, Arg b) Lys, Phe, Ser c) Lys d) Ala, Met, Ser4. CNBr treatment gives a) Ala, Arg, Lys2, homoserine, Phe, Ser b) Ala, Ser5. Thermolysin is a protease that cuts on the N-terminal side of hydrophobic amino acids (substrate preference is Leu, Ile, Phe, Trp, Tyr, Val). Thermolysin treatment of our polypeptide yields 2 peptides a) Ala, Arg, Ser b) Ala, Lys2, Met, Phe, Ser
- Select the term in column B which best matches the description in column A. There are more terms then needed. Column A Column B 1. The pentose sugar found in DNA. a) Cis fatty acid b) Trans fatty acid c) Coenzyme d) Cofacipr 2. Commonly referred to as "insoluble fiber". 3. Considered to be an amphipathic molecule. 4. A molecule with the formula C18H3602 is probably a e) Phospholipid 5. Bond created during the formation of the primary structure of a protein. ) Glycogen 6. A non-protein organic molecule needed for proper enzyme functioning. g) Cellulose 7. Pyrimidine base found only in RNA. h) Galactose i) Fructose 8. Increasing the number of these molecules in the cell membrane would increase the permeability of the cell membrane. i) Thymine k) Uracil 9. Bond which connects nucleotide monomers together. 1) Fatty acid m) Disaccharide n) Peptide bond o) A-Helix p) B-Pleated Sheet q) Ribose r) Deoxyribose 10.B. E- D C A Cell cytoplasm Cell cytoplasm Bacterial Cell A Bacterial Cell B A cross-section of the cell envelope of two bacterial cells, A and B, are shown in the figure. Which label is matched with the correct structure A) O Structure A: peptidoglycan B) O Structure B: teichoic acid C) O Structure C: lipoprotein D) O Structure D: capsule E) O Structure E: S layer Cell exterior Cell exteriorWhich of the following statements about electron microscopy are true? a) Most existing protein structures have been resolved using electron microscopy b) Electron microscopy is not used in structural biology as it can not give as high a resolution as X-ray crystallography and NMR c) The smaller a protein, the easier it is to solve its structure with electron microscopy d) By taking pictures of the same protein frozen in ice thousands of times and then adding them together, you get a high-resolution image of the protein e) The first protein structure with true atomic resolution was solved using electron microscopy last year
- If Kd= 100 nM, [RNA] = 10 nM, and [protein] = 1 uM a) What fraction of RNA is bound? b) What fraction of protein is bound? c) What is the concentration of the protein:RNA complex? d) Is it reasonable to assume that total protein = free protein? e) Is it reasonable to assume that total RNA = free RNA?Identify the following labeled structures.With regard to cytoskeletal structure and function, a) Outline the structure and organisation of cytoskeletal proteins in a eukaryotic, epithelial cell.
- You combine 0.2 g of yeast, 0.2 g of sucrose and 10 mL of water, place the mixture inside an uninfl ated balloon, then tie off the opening of the balloon. (a) Explain what you would observe over the next hour. (b) What would you observe if you added iodoacetate to the mixture? (c) What would you observe if the mixture contained lactose rather than sucrose?Differentiate between collagen, a-keratin and B-keratin and further tabulate in differences using a table in terms of: 1) structure 2) abundant amino acid 3) where it is found 4) commercial use 5) formation 6) diseases18.