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3.2. Consider the reversible product-formation reaction in an enzyme-catalyzed bioreaction: k (ES) E+P E+S: Develop a rate expression for product-formation using the quasi-steady-state approximation and shov that d[P] _ (v,/K„)[S]-(v,/K,)[P] m. v = dt [S] . [P] 1+ Km Kp k, +k, k, k_y +kg where Km and K, = and V, = k,[E,], V, =k¬[E,]. k_2

3.2. Consider the reversible product-formation reaction in an enzyme-catalyzed bioreaction: k (ES) E+P E+S: Develop a rate expression for product-formation using the quasi-steady-state approximation and shov that d[P] _ (v,/K„)[S]-(v,/K,)[P] m. v = dt [S] . [P] 1+ Km Kp k, +k, k, k_y +kg where Km and K, = and V, = k,[E,], V, =k¬[E,]. k_2

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Consider the reversible product-formation reaction in an enzyme–catalyzed bioreaction:

 

Develop a rate expression for product-formation using the quasi-steady-state approximation and show that

 

3.2. Consider the reversible product-formation reaction in an enzyme-catalyzed bioreaction: E+S: (ES) E+P Develop a rate expression for product-formation using the quasi-steady-state approximation and show that d[P] _ (v,/K„)[S]-(v,/K,)[P] % = dt [S] ¸ [P] 1+ + K m K, k+k2 and V, = k,[E,], V, =k¬[E,]. k_2 where Km k+k, 2 and Kp k,
Transcribed Image Text:3.2. Consider the reversible product-formation reaction in an enzyme-catalyzed bioreaction: E+S: (ES) E+P Develop a rate expression for product-formation using the quasi-steady-state approximation and show that d[P] _ (v,/K„)[S]-(v,/K,)[P] % = dt [S] ¸ [P] 1+ + K m K, k+k2 and V, = k,[E,], V, =k¬[E,]. k_2 where Km k+k, 2 and Kp k,
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