3. You want to determine the effectiveness of a novel cancer therapy, RS7, in treating lung cancer. You cultured lung cancer microtissues in cell culture plates with equal numbers of cells per well. You exposed plate 1 to the novel drug and left plate 2 untreated (control). You analyzed both plates using flow cytometry and propidium iodide to identify live and dead cells. The results below represent the number of viable cells. Using a t-test, determine whether RS7 reduces the viability of lung cancer cells. Plate 1 (RS7): X1 = 65,434 X2 = 70,250 Plate 2 (control): X'1 = 80,760 X'2 = 78,562 X3= 63,962 X'3=81,435 X4= 61,030 X'4 = 75,650 X5= 60,572 X'5 = 77,112 D) List the critical value for a two-tailed test from the t distribution table for df = (n1+n2-2) when n1 = n2 = 5 values/group and p = 0.05.

You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells in a tube with 5ml of culture medium. Then carried out a trypan blue assay and counted your cells with a hematocytometer as shown in Figure below. Answer the questions according to the results: a. Calculate the concentration of the stock including the dead and living cells. Dont forget to show the units! b. Calculate the percentage (%) of the living cells in the stock. c. You want to seed 6000 living cells into each well of 96 well plates, then calculate the volume you should take from the stock for each well. 輯 1 mm I IRE

Which of the following is false about counting viable cells?  A. CFU(Colony forming unit) are counted instead of individuals cells  B. Only 30-300 CFUs are statistically valid  C. Colonies growing on the plate represent a fraction of the live cells in the original sample  D. You can determine the number of live cells in a broth culture  E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sample

Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…