3. What is the structural requirement or a positive biuret test?4. When your fingers come in contact with nitric acid, it results in yellow stains. These stains can’t be removed by washing. Why? InstructionsUpon performing the xanthoproteic acid test, sulfur test, biuret test, and ninhydrin test, andprotein denaturation methods on various samples, the tables show the results of the experiment.The student is required to answer the questions relating to the data presented.Table A.Xanthoproteic TestSulfur TestAlbuminSlightly Opaque, OrangeSlightly opaque, brownCaseinSlightly Opaque, OrangeTransparent, light brown tintGelatinTransparent, YellowTransparent, ColorlessGlycineColorlessDistilled WaterColorlessTransparent, ColorlessCysteineOpaque, Black with ppt.TyrosineTransparent, YellowTryptophanTransparent, Dark OrangeTable B.Biuret TestNinhydrinTestAlbuminTransparent, PurpleOpaque, Dark blueCaseinSlightly opaque, Pink-purpleOpaque, Dark blueGelatinTransparent, Blue-PurpleTransparent, BlueGlycineClear, Light blueOpaque, Dark blueDistilled WaterClear, Light blueTransparent, ColorlessAlbuminGelatinDistilled WaterHeller's TestHeat without HC2H3O2+Picric Acid++Ethanol++HgCl2+

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Answered: 3. What is the structural requirement…

3. What is the structural requirement or a positive biuret test? 4. When your fingers come in contact with nitric acid, it results in yellow stains. These stains can’t be removed by washing. Why?

Complete Textbook Of Phlebotomy

5th Edition

ISBN:9781337464314

Author:Hoeltke

Publisher:Hoeltke

Chapter10: Sample Preparation And Handling

Section: Chapter Questions

Problem 3.1E

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Question

3. What is the structural requirement or a positive biuret test? 4. When your fingers come in contact with nitric acid, it results in yellow stains. These stains can’t be removed by washing. Why?

Instructions
Upon performing the xanthoproteic acid test, sulfur test, biuret test, and ninhydrin test, and
protein denaturation methods on various samples, the tables show the results of the experiment.
The student is required to answer the questions relating to the data presented.
Table A.
Xanthoproteic Test
Sulfur Test
Albumin
Slightly Opaque, Orange
Slightly opaque, brown
Casein
Slightly Opaque, Orange
Transparent, light brown tint
Gelatin
Transparent, Yellow
Transparent, Colorless
Glycine
Colorless
Distilled Water
Colorless
Transparent, Colorless
Cysteine
Opaque, Black with ppt.
Tyrosine
Transparent, Yellow
Tryptophan
Transparent, Dark Orange
Table B.
Biuret Test
NinhydrinTest
Albumin
Transparent, Purple
Opaque, Dark blue
Casein
Slightly opaque, Pink-purple
Opaque, Dark blue
Gelatin
Transparent, Blue-Purple
Transparent, Blue
Glycine
Clear, Light blue
Opaque, Dark blue
Distilled Water
Clear, Light blue
Transparent, Colorless
Albumin
Gelatin
Distilled Water
Heller's Test
Heat without HC2H3O2
+
Picric Acid
+
+
Ethanol
+
+
HgCl2
+

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