3. What is the structural requirement or a positive biuret test? 4. When your fingers come in contact with nitric acid, it results in yellow stains. These stains can’t be removed by washing. Why?
3. What is the structural requirement or a positive biuret test? 4. When your fingers come in contact with nitric acid, it results in yellow stains. These stains can’t be removed by washing. Why?
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.13: Erythrocyte Sedimentation Rate
Problem 1CS
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3. What is the structural requirement or a positive biuret test?
4. When your fingers come in contact with nitric acid, it results in yellow stains. These stains can’t be removed by washing. Why?
Transcribed Image Text:Instructions
Upon performing the xanthoproteic acid test, sulfur test, biuret test, and ninhydrin test, and
protein denaturation methods on various samples, the tables show the results of the experiment.
The student is required to answer the questions relating to the data presented.
Table A.
Xanthoproteic Test
Sulfur Test
Albumin
Slightly Opaque, Orange
Slightly opaque, brown
Casein
Slightly Opaque, Orange
Transparent, light brown tint
Gelatin
Transparent, Yellow
Transparent, Colorless
Glycine
Colorless
Distilled Water
Colorless
Transparent, Colorless
Cysteine
Opaque, Black with ppt.
Tyrosine
Transparent, Yellow
Tryptophan
Transparent, Dark Orange
Table B.
Biuret Test
NinhydrinTest
Albumin
Transparent, Purple
Opaque, Dark blue
Casein
Slightly opaque, Pink-purple
Opaque, Dark blue
Gelatin
Transparent, Blue-Purple
Transparent, Blue
Glycine
Clear, Light blue
Opaque, Dark blue
Distilled Water
Clear, Light blue
Transparent, Colorless
Albumin
Gelatin
Distilled Water
Heller's Test
Heat without HC2H3O2
+
Picric Acid
+
+
Ethanol
+
+
HgCl2
+
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