3. What is the structural requirement or a positive biuret test? 4. When your fingers come in contact with nitric acid, it results in yellow stains. These stains can’t be removed by washing. Why?

Complete Textbook Of Phlebotomy
5th Edition
ISBN:9781337464314
Author:Hoeltke
Publisher:Hoeltke
Chapter10: Sample Preparation And Handling
Section: Chapter Questions
Problem 3.1E
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3. What is the structural requirement or a positive biuret test? 4. When your fingers come in contact with nitric acid, it results in yellow stains. These stains can’t be removed by washing. Why?
Instructions
Upon performing the xanthoproteic acid test, sulfur test, biuret test, and ninhydrin test, and
protein denaturation methods on various samples, the tables show the results of the experiment.
The student is required to answer the questions relating to the data presented.
Table A.
Xanthoproteic Test
Sulfur Test
Albumin
Slightly Opaque, Orange
Slightly opaque, brown
Casein
Slightly Opaque, Orange
Transparent, light brown tint
Gelatin
Transparent, Yellow
Transparent, Colorless
Glycine
Colorless
Distilled Water
Colorless
Transparent, Colorless
Cysteine
Opaque, Black with ppt.
Tyrosine
Transparent, Yellow
Tryptophan
Transparent, Dark Orange
Table B.
Biuret Test
NinhydrinTest
Albumin
Transparent, Purple
Opaque, Dark blue
Casein
Slightly opaque, Pink-purple
Opaque, Dark blue
Gelatin
Transparent, Blue-Purple
Transparent, Blue
Glycine
Clear, Light blue
Opaque, Dark blue
Distilled Water
Clear, Light blue
Transparent, Colorless
Albumin
Gelatin
Distilled Water
Heller's Test
Heat without HC2H3O2
+
Picric Acid
+
+
Ethanol
+
+
HgCl2
+
Transcribed Image Text:Instructions Upon performing the xanthoproteic acid test, sulfur test, biuret test, and ninhydrin test, and protein denaturation methods on various samples, the tables show the results of the experiment. The student is required to answer the questions relating to the data presented. Table A. Xanthoproteic Test Sulfur Test Albumin Slightly Opaque, Orange Slightly opaque, brown Casein Slightly Opaque, Orange Transparent, light brown tint Gelatin Transparent, Yellow Transparent, Colorless Glycine Colorless Distilled Water Colorless Transparent, Colorless Cysteine Opaque, Black with ppt. Tyrosine Transparent, Yellow Tryptophan Transparent, Dark Orange Table B. Biuret Test NinhydrinTest Albumin Transparent, Purple Opaque, Dark blue Casein Slightly opaque, Pink-purple Opaque, Dark blue Gelatin Transparent, Blue-Purple Transparent, Blue Glycine Clear, Light blue Opaque, Dark blue Distilled Water Clear, Light blue Transparent, Colorless Albumin Gelatin Distilled Water Heller's Test Heat without HC2H3O2 + Picric Acid + + Ethanol + + HgCl2 +
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