EFFECT OF ENZYMECONCENTRATION ONENZYME ACTIVITYProcedure:1. Prepare 5 test tubes. Place 1 ml of 1%starch and add 10 drops of saliva toeach tube. Mix thoroughly.2. Place the first tube in ice water, the 2ndtube leave at room temperature, the 3rdtube in 400C, the 4thtube at 60oCwater bath and the 5thtube boil for 2minutes..3. Leave the 4 tubes in their respectivetemperatures for 30 minutes. The 4thtubeallows to stand for 30 minutes after heatingfor 2 minutes.4. Test the contents of each tube with iodineand benedict's tests.a. lodine Test – to half of the content of eachtube, add 1 drop of iodine in KIand note the color produced. Compare theintensity of color in each of the6 tubes.b. Benedict's – to 1 ml benedict's reagent,-add 5 drops of the other half ofeach tube and heat in a boiling water bathfor 3 minutes. Note color ofprecipitate 3. What is the effect of temperature below and above the optimum temperature on reactionrate?

Enzymes are the molecules that catalyse the conversion of substrate molecules to products by…

Answered: 3. What is the effect of temperature…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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2. Draw a graph showing a downhill (spontaneous) reaction, that requires some activation energy to get it going. Then draw and clearly label on the graph b. The activation energy for the reaction (use a bracket to indicate the upper and lower limits) c. The free energy change for the reactions (use a bracket to indicate the upper and lower limits) d. How an enzyme will change the energetics of the reaction. I suggest you use a dotted line for the enzyme.
1. A student was given two test tubes. One tube contains a reaction catalyzed by an inorganic catalyst, while the second tube contains a reaction catalyzed by an enzyme which is protein in nature. Give two laboratory procedures which you can perform to differentiate which of the two tubes contain an enzyme-catalyzed reaction.
8 eBook Select all of the following that could apply to the brown curve. Enzyme concentration is higher. Temperature for an enzyme is too high. Temperature for an enzyme is optimal. Activation energy is lower. Enzyme concentration is lower. Activation energy is higher. Reactants Progress of reaction, Products
Choose the correct effect. * increase decrease not change Raising the temperature slightly will... the rate of reaction Boiling temperature will... the rate of reaction Changing the pH toward the optimal pH will... the rate of reaction Introducing a competitive inhibitor will... the rate of reaction
How do chemical and nuclear reactions differ in(a) Magnitude of the energy change?(b) Effect on rate of increasing temperature?(c) Effect on rate of higher reactant concentration?(d) Effect on yield of higher reactant concentration?
5. Draw the reaction curve for the follow a) A reaction with an activation energy (EA) = 50 and free energy ( G) = 20 %3D %3D b) A reaction with an activation energy (EA) = 30 and free energy ( G) = - 40 c) Indicate whether the reactions above are anabolic or catabolic Reaction a) %D Reaction b) = %3D
5. Give the major product of the following reaction. OH H+, water, heat
20) Normally an enzyme has maximal enzyme activity at 37°C, but once it has been heated to 100°C and then cooled back down to 37°C the enzyme is no longer active. Why?
(i)Define initial velocity (ii) Describe how you would calculate initial velocity from a graph of product concentration against time for a chemical reaction
1. Answer the following questions, based on this reaction: S+E P ES EP2 E + P | B)Where is the transition state located?
1. Why do we need to repeat the measurements of reaction of times ten times?
The following questions are based on the reaction A+ B ↔ C+D shown in Figure 8.1. 1. Which of the following terms best describes the progress of the reaction with respect to free energy change? a) endergonic, ∆G> 0 b) exergonic, ∆G> 0 c) exergonic, ∆G< 0 d) endergonic, ∆G< 0 2. Which of the following in Figure 8.1 remains unchanged by having an enzyme included? a) b b) d c) a d) c 3. The part labeled “C” on the above graph represents a) Energy of activation without enzyme b) Energy of activation with enzyme c) Amount of free energy released d) amount of energy required for the reaction progress

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3. What is the effect of temperature below and above the optimum temperature on reaction rate?