3. Relation between Reaction Velocity and Substrate Concentration: Michaelis-Menten Equation (a) At what substrate concentration would an enzyme with a k of 30.0 s¹ cat and a K of 0.0050M operate at one-quarter of its maximum rate? m Vo/Vmax=0.25 (b) Determine the fraction of that would be obtained at the following substrate concentrations [S]: ½ K, 2 K, and 10 K. m max (c) An enzyme that catalyzes the reaction XY is isolated from two bacterial species. The enzymes have the same Vmax, but different Km values for the substrate X. Enzyme A has a Km of 2.0 M, while enzyme B has a Km of 0.5 M. The plot below shows the kinetics of reactions carried out with the same concentration of each enzyme and with [X] = 1 [M. Which curve corresponds to which enzyme? [Y] Time
3. Relation between Reaction Velocity and Substrate Concentration: Michaelis-Menten Equation (a) At what substrate concentration would an enzyme with a k of 30.0 s¹ cat and a K of 0.0050M operate at one-quarter of its maximum rate? m Vo/Vmax=0.25 (b) Determine the fraction of that would be obtained at the following substrate concentrations [S]: ½ K, 2 K, and 10 K. m max (c) An enzyme that catalyzes the reaction XY is isolated from two bacterial species. The enzymes have the same Vmax, but different Km values for the substrate X. Enzyme A has a Km of 2.0 M, while enzyme B has a Km of 0.5 M. The plot below shows the kinetics of reactions carried out with the same concentration of each enzyme and with [X] = 1 [M. Which curve corresponds to which enzyme? [Y] Time
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Please Explain each part to the best of your ability. This is Biochemistry. Thank you
![3. Relation between Reaction Velocity and Substrate Concentration:
Michaelis-Menten Equation
(a) At what substrate concentration would an enzyme with a k of 30.0 s¹
cat
and a K of 0.0050M operate at one-quarter of its maximum rate?
m
Vo/Vmax=0.25
(b) Determine the fraction of that would be obtained at the following
substrate concentrations [S]: ½ K, 2 K, and 10 K.
m
max
(c) An enzyme that catalyzes the reaction XY is isolated from two
bacterial species. The enzymes have the same Vmax, but different Km
values for the substrate X. Enzyme A has a Km of 2.0 M, while enzyme
B has a Km of 0.5 M. The plot below shows the kinetics of reactions
carried out with the same concentration of each enzyme and with [X] = 1
M. Which curve corresponds to which enzyme?
[Y]
Problem 6-8
Lehninger Principles of Biochemistry, Fifth Edition
©2008 WH Freeman and Company
Time](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0e605900-2fca-4b5f-8414-bb83c48d1fa4%2F93b695c9-25b9-4a60-926f-f8b60c5cc5b8%2Fc9yp3g4_processed.jpeg&w=3840&q=75)
Transcribed Image Text:3. Relation between Reaction Velocity and Substrate Concentration:
Michaelis-Menten Equation
(a) At what substrate concentration would an enzyme with a k of 30.0 s¹
cat
and a K of 0.0050M operate at one-quarter of its maximum rate?
m
Vo/Vmax=0.25
(b) Determine the fraction of that would be obtained at the following
substrate concentrations [S]: ½ K, 2 K, and 10 K.
m
max
(c) An enzyme that catalyzes the reaction XY is isolated from two
bacterial species. The enzymes have the same Vmax, but different Km
values for the substrate X. Enzyme A has a Km of 2.0 M, while enzyme
B has a Km of 0.5 M. The plot below shows the kinetics of reactions
carried out with the same concentration of each enzyme and with [X] = 1
M. Which curve corresponds to which enzyme?
[Y]
Problem 6-8
Lehninger Principles of Biochemistry, Fifth Edition
©2008 WH Freeman and Company
Time
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