3. In the DNA segment5'-ATGAGGCATGAGACG-3' (coding strand) 3'-TACTCCGTACTCTGC-5'(template strand)• What products would be fomed from the segment's replication?• Write the MRNA sequence that would be obtained from the segment's transcription.What is the amino acid sequence of the peptide produced from the mRNA?

Deoxyribonucleic acid (DNA) contains 2 segments referred to as coding and template strand. These…

Answered: 3. In the DNA segment…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Based on your understanding of transcription, place the following events in the correct order. 1. A base pairs with U, and G with C 2. RNA released 3. RNA polymerase binds 4. transcription bubble 5. DNA polymerase grows new chain 6. A base pairs with T, and C with G 7. Promoter is reached O 3,4, 7, 5, 6, 1 O 5, 6, 3, 1, 2 O 7,3, 4, 6, 1, 2 O 3, 7, 4, 1, 2 O 5, 2, 4, 7, 1, 3
2. List the DNA strand sequence complementary to the template strand. CCUGCAGUAUGAAACGCCUGGUAGAAGGUGGGAAGUGGUGCGCCC . . .
How is transcription different in eukaryotes compared to prokaryotes? Choose one: • Transcription occurs in the nucleus in eukaryotes and in the cytoplasm in prokaryotes. Eukaryotes transcribe both strands of DNA, while prokaryotes transcribe only one strand. Eukaryotes use RNA polymerase for transcription, while prokaryotes use ribosomes.
5. Design a 10-bp primer that could be used to amplify the following sequence of DNA: 5'-AGTCGATCCCTGATCGTACGCTACGGTAACGT-3'
9. Identify the TRUE statement about the process of transcription: * ORNA polymerase requires an RNA primer molecule before it can begin transcript ODNA is not needed for the process of transcription. RNA polymerase binds to a promoter region in the DNA to initiate transcription. O During the elongation phase, RNA transcripts are built from 3' to 5'. 10. Which is correct? * Translation occurs in the nucleus; transcription occurs in the cytoplasm O Transcription occurs in the nucleus; translation occurs in the cytoplasm Both transcription and translation take place in the cytoplasm Both transcription and translation occur in the nucleus
Exercise In this exercise we will practice transcribing and translating sample DNA. Using your sample DNA, unzipped and second strand removed, you will first create an RNA strand (transcription). DNA is read in the 5' → 3' direction, so when you create RNA, a 3'end pairs with the 5' end of DNA. From your RNA strand, you will need to create codons; remember that a codon is a group of 3 bases that codes for a specific amino acid. Your codons are read in the 5' → 3' direction (hint: it might be right to left!). You will then need to convert your codons into amino acids in the 5' → 3' direction (translation). DNA strand 3' TAC-TTA-CGA-TGG-TAC-ACG-CAA-TCT-ATA-CTC-AAA-TAT-AGG-ACC-TTG-ACG-TCG-AAT-CTC-CAC-TGT-ACC-TTG-AAC-CTG-ACT 5' RNA strand 5'-AUG-AAU Amino acid sequence G ne (9)
3. Differentiate between factors and processes involved in transcription: Recognition of this DNA sequence is [Choose] required for transcription to initiate:. [ Choose ] PROMOTER Occurs when the ribosome reaches a RNA POLYMERASE stop codon on the MRNA TERMINATION INITIATION SITE ENHANCERS The main enzyme responsible for transcribing DNA into RNA is ELONGATION Will increase the efficiency of gene [Choose] expression, but are not essential for transcription. RNA polymerase "walks" along the template strand, adding a [Choose ] matching (complementary) RNA nucleotide to each existing ncleotide. The section of DNA from which the [Choose ) very first 5" RNA nucleotide is transcribed Question 4 1 pts hp 近

3. A mutation was introduced to the original DNA and now you have following DNA sequence (the mutation is colored in red.). 5' -GGATGCATGCCCCTCAAAGGGTAAATT-3' (coding strand) 3' -CCTACGTACGGGGAGTTTCCCATTTAA-5' (template strand) What is going to happen to the protein coded by this gene? Write expected amino acid sequence (like Pro-Arg-Tyr-)

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