3. Carbonic anhydrase has two substrates, carbon dioxide and bicarbonate, which are both converted to carbonic acid. Kinetic data for each is given below. While determining the kinetics of HCO3 as a substrate, how would the addition of CO2 effect the reaction if the rate were measured by the disappearance of bicarbonate? Substrate Km (mM) Kcat (sec 1) Kcat/Km (m M-1sec 1) CO2 12 1x106 8.3x104 HCO3 26 4x105 1.5x104 4. The Cleland shorthand for two different enzyme mechanisms that use two suhstrater

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3. Carbonic anhydrase has two substrates, carbon dioxide and bicarbonate, which are both converted to carbonic acid. Kinetic data for each is
given below. While determining the kinetics of HCO3 as a substrate, how would the addition of CO2 effect the reaction if the rate were
measured by the disappearance of bicarbonate?
Substrate
Km (mM)
Kcat (sec 1)
Kcat/Km (m M-1sec 1)
CO2
12
1x106
8.3x104
HCO3
26
4x105
1.5x104
4. The Cleland shorthand for two different enzyme mechanisms that use two suhstrater and
Transcribed Image Text:3. Carbonic anhydrase has two substrates, carbon dioxide and bicarbonate, which are both converted to carbonic acid. Kinetic data for each is given below. While determining the kinetics of HCO3 as a substrate, how would the addition of CO2 effect the reaction if the rate were measured by the disappearance of bicarbonate? Substrate Km (mM) Kcat (sec 1) Kcat/Km (m M-1sec 1) CO2 12 1x106 8.3x104 HCO3 26 4x105 1.5x104 4. The Cleland shorthand for two different enzyme mechanisms that use two suhstrater and
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