3. A particular protein heat denatures with a melting temperature Tm = 332 K with an enthalpy of unfolding, AHm = 372 kJmol-¹. (a) What is the entropy of unfolding in kJ mol-1 K-1 at Tm? (b) If ACp is 6.6 kJ mol-1 K-1, at what temperature does the free energy of unfolding (AG unfolding) have the greatest value (i.e. the native state is most stable)? (c) Calculate the value of AG and K at this temperature.

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3. A particular protein heat denatures with a melting temperature Tm = 332 K with
an enthalpy of unfolding, AHm = 372 kJmol-¹.
(a) What is the entropy of unfolding in kJ mol-1 K-1 at Tm?
(b) If ACp is 6.6 kJ mol-1 K-1, at what temperature does the free energy
of unfolding (AG unfolding) have the greatest value (i.e. the native
state is most stable)?
(c) Calculate the value of AG and K at this temperature.
Transcribed Image Text:3. A particular protein heat denatures with a melting temperature Tm = 332 K with an enthalpy of unfolding, AHm = 372 kJmol-¹. (a) What is the entropy of unfolding in kJ mol-1 K-1 at Tm? (b) If ACp is 6.6 kJ mol-1 K-1, at what temperature does the free energy of unfolding (AG unfolding) have the greatest value (i.e. the native state is most stable)? (c) Calculate the value of AG and K at this temperature.
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