2. Your lab mate, Beaker, tells you that they have run into some issues with expressing a gene on a plasmid they've recently created. The gene has a proper promoter and a lacO sequence and no lacl sequence on the plasmid so that there will be constitutive expression of the gene. Beaker used standard protocols to transform wild-type E. coli with this plasmid and verified the colony they chose has the plasmid with the gene. However, Beaker cannot seem to recover any gene product (MRNA or protein) from a standard culture with appropriate media. What has your lab mate done wrong with this experiment? . What options are there for your lab mate to fix this issue? There are three possible options, specify two options and justify them.
2. Your lab mate, Beaker, tells you that they have run into some issues with expressing a gene on a plasmid they've recently created. The gene has a proper promoter and a lacO sequence and no lacl sequence on the plasmid so that there will be constitutive expression of the gene. Beaker used standard protocols to transform wild-type E. coli with this plasmid and verified the colony they chose has the plasmid with the gene. However, Beaker cannot seem to recover any gene product (MRNA or protein) from a standard culture with appropriate media. What has your lab mate done wrong with this experiment? . What options are there for your lab mate to fix this issue? There are three possible options, specify two options and justify them.
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications
Section: Chapter Questions
Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...
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