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Given that the initial concentration is .2 M and volume is 575 ml .
We have to find concentration after diluting it with 600 ml .
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- Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.1. Calculate the pH of a buffer solution made from 0.20 M CH3COOH and 0.50 M CH3COO- that has an acid dissociation constant for CH3COOH of 1.8 x 10-5.1. Describe how you would go about making up the following solutions. a) 150ml GTE buffer (resuspension solution) containing, 20mM Tris pH 8, 5000µM EDTA, 1M Glucose
- 2. Calculate the pH of a buffer containing 0.1 M sodium acetate and 0.2 M acetic acid (pKa = 4.76).If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.1. What volumes of 50 mM CH3COOH and 30 mM NaCH3CO0 solutions are needed to prepare 2L of 20 mM acetate buffer pH 3.6? (pka for acetic acid is 4.74)
- How much of a 10x buffer should be added to 900ml of water to make a 1x solution?6. Of the following pairs write which ones could be used as buffers and why: (a) 0.1 M NaOH and 0.1 M NaCH3COO, (b) 0.01 M H2SO4 and 0.01 M K2S04, (c) 0.01 M NH3 and 0.02 M NH4CI, (d) 0.2 M HNO2 and 0.2 M NaNO2.2. If it is assumed that a sample of pure Sodium Carbonate weighing 2.6250g required 60.20mL of the acid in the titration. What is the normality of the acid?
- 6. What volume of a 9% NaCl solution is needed to prepare 100 mL of a 0.9% NaCl solution?16. How many milliliters of a 0.9% (w/v) solution of sodium chloride can be pre- pared from 50 tablets, each containing 2.25 g of sodium chloride? 16. 12,500 mL25. What would be the new pH of a 1L buffer solution (pH=7.8) upon the addition of 3mL 0.0001 M HCI. (Buffer, pka=7.2) * A.) 4.9 O B.) 10.0 O C.) 3.0 O D.) 0.0001 O E.) No detectable effect