2. Separation of Amino Acids by lon-Exchange Chromatography Mixtures of amino acids can beanalyzed by first separating the mixture into its components through ion-exchange chromatography. Amino acids placed on a cation-exchange resin (see Fig. 3-17a) containing sulfonate (OSO3_) groups slow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column and positively charged functional groups on the amino acids, and (2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin. For each pair of amino acids listed, determine which will be eluted first from an ion-exchange column by a pH 7.0 buffer. (a) Asp and Lys (b) Arg and Met (c) Glu and Val (d) Gly and Leu (e) Ser and Ala
2. Separation of Amino Acids by lon-Exchange Chromatography Mixtures of amino acids can beanalyzed by first separating the mixture into its components through ion-exchange chromatography. Amino acids placed on a cation-exchange resin (see Fig. 3-17a) containing sulfonate (OSO3_) groups slow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column and positively charged functional groups on the amino acids, and (2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin. For each pair of amino acids listed, determine which will be eluted first from an ion-exchange column by a pH 7.0 buffer. (a) Asp and Lys (b) Arg and Met (c) Glu and Val (d) Gly and Leu (e) Ser and Ala
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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***not graded, just a practice problem I do not understand. Thank you so much!

Transcribed Image Text:### Separation of Amino Acids by Ion-Exchange Chromatography
Mixtures of amino acids can be analyzed by first separating the mixture into its components through ion-exchange chromatography. Amino acids placed on a cation-exchange resin (see Fig. 3-17a) containing sulfonate (OSO₃⁻) groups slow down the column at different rates because of two factors that influence their movement:
1. **Ionic Attraction:** This occurs between the sulfonate residues on the column and the positively charged functional groups on the amino acids.
2. **Hydrophobic Interactions:** This occurs between the amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin.
Given these two factors, for each pair of amino acids listed below, determine which will be eluted first from an ion-exchange column by a pH 7.0 buffer:
- (a) Asp and Lys
- (b) Arg and Met
- (c) Glu and Val
- (d) Gly and Leu
- (e) Ser and Ala
### Explanation of Diagram (Fig. 3-17a)
*Note: The actual diagram referenced is not visible. Here’s a general description:*
**Figure 3-17a:** This is an illustration of a cation-exchange column used for separating amino acids. The diagram would typically show a column packed with a resin containing sulfonate (OSO₃⁻) groups. The amino acids are introduced at the top of the column, and their journey through the column is slowed based on their ionic and hydrophobic interactions with the resin. Typically, the diagram might also indicate the point where the amino acids are introduced, the direction of flow of the buffer solution, and the points where different amino acids elute from the column. The elution order depends on the amino acids' relative affinities for the ion-exchange resin and their interactions with the column material.
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