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- 10. A single cell is placed into a culture tube containing nutrient agar. If the number of cells triples every 2 minutes and the culture tube is completely filled in 12 hours, how long does it take for the culture tube to be only half full of cells?13. A certain bacteria can grow 10-fold every 3 hours. If there are 500 bacteria now, express number of bacteria "n" as a function of elapsed time in hours. a) Determine the equation of bacteria growth as a function of time. b) Draw a chart with intervals of 3 hours, where 0 Shs 18.Im 100 4. You have a culture of yeast that is at a density of 8x10' cells/mL. You dilute the sample 1:10, then 1:1,000 again, and finally you dilute the sample an additional 1:5. You add 0.1 mL of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many colony- forming units do you expect to count on your pour plate the next day?
- How long would it take a single bacterial cell to form 1,000,000 cells if it had a generation time of 30 minutes?In a certain culture of bacteria, the rate of increase is proportional to the number present. (a) If it is found that the number doubles in 6 hrs, how many may be expected at the end of 18 hrs? (b) If there are 102 at the end of 4 hrs, and 8 ∙ 102 at the end of 8 hrs, how many were there in the beginning?4. You have a culture of yeast that is at a density of 8x107 cells/mL. You dilute the sample 1:10, then 1:1,000 again, and finally you dilute the sample an additional 1:5. You add 0.1 mL of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many colony- forming units do you expect to count on your pour plate the next day?
- 17 Which of the following best described the graph shown: Log (number of bacteria) Bacterial Growth Curve TimeYou are incubating cells for 3 hours. The initial number of cells present in the sample were 102. After the incubation period, you find that the culture now has 104 cells. What is the generation time in minutes?Why would you want to limit the number of times you passage a cell culture line?
- A bacteria having a generation time of 30 minutes will undergo 6 cell division/hr. During 6 hrs of growth, the cells would double 5 times. Beginning with 20 cells/mL at time 0, how many cells would be present at 5 hrs?If a bacterium has a generation time of 30 minutes, and you start with 100 cells at time 0, how many cells will you have in 30, 60, 90, and 120 minutes?9. You make a 2 ml starter culture of a strain of E coli you typically work with. You take 100 pl of this starter culture and inoculate flask containing 1 liter of TSA broth and incubate it for 20 hours. In the meantime you take 10 µl of this culture and do serial dilutions to determine the number of cells that you inoculated the large culture with. What is the concentration of E coli? 10 pl PBS 50 ul 50 ul 50 ul 50 µl 50 ul P 450 μι PBS 450 ul PBS 450 ul PBS 450 ul PBS 450 ul PBS 990 µl Cell lysate PBS 10-1 dilution 10-2 dilution 10-3 dilution 10-4 dilution 10-5 dilution Starter Culture 125 CFUS Worksheet 4 Biol235L Sp21 10. Assuming a doubling time of 20 minutes for exponentially growing E coli, how many cells do you end up with?