2. Birds have evolved over the last 60-70 MY. To learn about their relatedness, we can use DNA sequences. Because DNA evolves at a relatively steady rate, gene sequences in two different organisms will accumulate sequence changes and become more different with time. The longer ago their common ancestor was, the more different their sequences will be. However, some genes are under more selection and so evolve slowly. Other genes are under less selection and so evolve quite quickly. Assuming that sites change linearly with time, we can calculate how many sites differ when comparing genes between different species. For simplicity we'll assume we are comparing 1000bp of sequence for each of these genes. Species compared Canary and olive finch Canary and house finch Canary and zebra finch Canary and robin Canary and chicken a. Why do the three genes differ in the number of variable sites between different species? # of sites that vary when comparing 2 sequences Elongation factor la Green sensitive opsin Mitochondrial cytb 19 34 84 106 235 1 1 Canary Olive finch House finch Zebra finch robin chicken 3 4 10 Here is some (hypothetical) data that shows the fraction of sites that differ when comparing the green sensitive opsin gene across all possible pairs of the different species. Olive finch House finch 0.008 0.000 0.014 0.035 0.045 0.097 Zebra finch 0.014 0.014 0.000 Canary 0.000 0.035 0.008 0.035 0.014 0.035 0.035 0.035 0.000 0.044 0.042 0.043 0.098 0.096 0.099 Sketch a simple tree that shows how these organisms are related to each other, based on this data. Robin d. Why would the cyt b gene do a poor job of determining bird relationships? e. Why is the green sensitive opsin gene just right in this case? 160 280 700 750 750 0.044 0.045 0.042 0.043 0.000 0.100 Chicken 0.098 0.097 0.096 0.099 0.100 0.000 c. Why would the elongation factor la gene do a poor job of determining the relationships of these birds?

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2. Birds have evolved over the last 60-70 MY. To learn about their relatedness, we can use DNA
sequences. Because DNA evolves at a relatively steady rate, gene sequences in two different
organisms will accumulate sequence changes and become more different with time. The longer ago
their common ancestor was, the more different their sequences will be. However, some genes are
under more selection and so evolve slowly. Other genes are under less selection and so evolve quite
quickly. Assuming that sites change linearly with time, we can calculate how many sites differ when
comparing genes between different species. For simplicity we'll assume we are comparing 1000bp of
sequence for each of these genes.
Species compared
Canary and olive finch
Canary and house finch
Canary and zebra finch
Canary and robin
Canary and chicken
a. Why do the three genes differ in the number of variable sites between different species?
Canary
Olive finch
House finch
Zebra finch
robin
chicken
Canary
# of sites that vary when comparing 2 sequences
Elongation factor la Green sensitive opsin Mitochondrial cytb
19
34
0.000
0.008
0.014
0.035
0.044
0.098
1
1
3
4
10
Here is some (hypothetical) data that shows the fraction of sites that differ when comparing the green
sensitive opsin gene across all possible pairs of the different species.
Olive finch
0.008
0.000
0.014
0.035
0.045
0.097
House
finch
84
106
235
0.014
0.014
0.000
0.035
0.042
0.096
Zebra
finch
0.035
0.035
0.035
0.000
0.043
0.099
Robin
160
280
700
750
750
0.044
0.045
0.042
0.043
0.000
0.100
d. Why would the cyt b gene do a poor job of determining bird relationships?
e. Why is the green sensitive opsin gene just right in this case?
Chicken
0.098
0.097
0.096
0.099
0.100
0.000
Sketch a simple tree that shows how these organisms are related to each other, based on this data.
c. Why would the elongation factor la gene do a poor job of determining the relationships of these
birds?
Transcribed Image Text:2. Birds have evolved over the last 60-70 MY. To learn about their relatedness, we can use DNA sequences. Because DNA evolves at a relatively steady rate, gene sequences in two different organisms will accumulate sequence changes and become more different with time. The longer ago their common ancestor was, the more different their sequences will be. However, some genes are under more selection and so evolve slowly. Other genes are under less selection and so evolve quite quickly. Assuming that sites change linearly with time, we can calculate how many sites differ when comparing genes between different species. For simplicity we'll assume we are comparing 1000bp of sequence for each of these genes. Species compared Canary and olive finch Canary and house finch Canary and zebra finch Canary and robin Canary and chicken a. Why do the three genes differ in the number of variable sites between different species? Canary Olive finch House finch Zebra finch robin chicken Canary # of sites that vary when comparing 2 sequences Elongation factor la Green sensitive opsin Mitochondrial cytb 19 34 0.000 0.008 0.014 0.035 0.044 0.098 1 1 3 4 10 Here is some (hypothetical) data that shows the fraction of sites that differ when comparing the green sensitive opsin gene across all possible pairs of the different species. Olive finch 0.008 0.000 0.014 0.035 0.045 0.097 House finch 84 106 235 0.014 0.014 0.000 0.035 0.042 0.096 Zebra finch 0.035 0.035 0.035 0.000 0.043 0.099 Robin 160 280 700 750 750 0.044 0.045 0.042 0.043 0.000 0.100 d. Why would the cyt b gene do a poor job of determining bird relationships? e. Why is the green sensitive opsin gene just right in this case? Chicken 0.098 0.097 0.096 0.099 0.100 0.000 Sketch a simple tree that shows how these organisms are related to each other, based on this data. c. Why would the elongation factor la gene do a poor job of determining the relationships of these birds?
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