2. A spectrophotometric method for the quantitative determination of the concentration of Pb in blood uses Cu* as an internal standard. A solution was prepared by combining 2.50mL of 7.000ppb Pb* with 1.000ml of 22.50ppb Cu the mixture was then diluted to 10.00mL. Which give an absorbance signal of 3.20 for lead and 1.35 for the copper. A 5.00mL sample of blood with unknown concentration of Pb?* is spiked with 1.000ml of 22.5ppb Cu and diluted to 10.00mL the resulting solution gave a signal of 4.95 for lead and 2.75 for copper. What is the concentration of lead in the blood sample?

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2. A spectrophotometric method for the quantitative determination of the concentration of Pb in
blood uses Cu?* as an internal standard. A solution was prepared by combining 2.50ml of 7.000ppb Pb?*
with 1.000mL of 22.50ppb Cu? the mixture was then diluted to 10.00mL. Which give an absorbance
signal of 3.20 for lead and 1.35 for the copper. A 5.00mLl sample of blood with unknown concentration
of Pb?* is spiked with 1.000ml of 22.5ppb Cu? and diluted to 10.00mL the resulting solution gave a
signal of 4.95 for lead and 2.75 for copper. What is the concentration of lead in the blood sample?
Transcribed Image Text:2. A spectrophotometric method for the quantitative determination of the concentration of Pb in blood uses Cu?* as an internal standard. A solution was prepared by combining 2.50ml of 7.000ppb Pb?* with 1.000mL of 22.50ppb Cu? the mixture was then diluted to 10.00mL. Which give an absorbance signal of 3.20 for lead and 1.35 for the copper. A 5.00mLl sample of blood with unknown concentration of Pb?* is spiked with 1.000ml of 22.5ppb Cu? and diluted to 10.00mL the resulting solution gave a signal of 4.95 for lead and 2.75 for copper. What is the concentration of lead in the blood sample?
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