Heat soparates the double strands of the original DNA into two single strands. 2 As the mixture cools, the primers pair with the original DNA. The same three-step cycle can be repeated many times, yielding billions of copies of the original DNA. New DNA en Original DNA sequence Primer New DNA New DNA - Le New DNA DNA polymerase fills in the missing nucleotides, producing new copies of the original DNA. Cycle 1 Cycle 2 Cycle 3

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Q1: PCR replicates DNA many times to increase the amount available for analysis. Why is this process called “amplification”? Q2: During the PCR cycle, what causes the DNA strands to separate? Q3: Identify a difference between how PCR and DNA replication are accomplished.

Heat soparates
the double
strands of the
original DNA
into two single
strands.
2 As the
mixture cools,
the primers
pair with the
original DNA.
The same three-step cycle can be
repeated many times, yielding
billions of copies of the original DNA.
New DNA
en
Original DNA
sequence
Primer
New DNA
New DNA
-
Le
New DNA
DNA polymerase fills in the
missing nucleotides, producing
new copies of the original DNA.
Cycle 1
Cycle 2
Cycle 3
Transcribed Image Text:Heat soparates the double strands of the original DNA into two single strands. 2 As the mixture cools, the primers pair with the original DNA. The same three-step cycle can be repeated many times, yielding billions of copies of the original DNA. New DNA en Original DNA sequence Primer New DNA New DNA - Le New DNA DNA polymerase fills in the missing nucleotides, producing new copies of the original DNA. Cycle 1 Cycle 2 Cycle 3
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