13. Consider the TLC plate below to answer the question. What is the Rf of the top component? Note: the distance between the origin and the bottom spot is 1.4 cm. The distance between the two spots is 1.6 cm. Solvent front 7.2 8.4 1.6 Baseline
13. Consider the TLC plate below to answer the question. What is the Rf of the top component? Note: the distance between the origin and the bottom spot is 1.4 cm. The distance between the two spots is 1.6 cm. Solvent front 7.2 8.4 1.6 Baseline
Chemistry
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Chapter1: Chemical Foundations
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Transcribed Image Text:### Thin Layer Chromatography Analysis
**Question 13: TLC Plate Analysis**
Consider the TLC plate illustration to answer the following question. What is the Rf value of the top component?
#### Description:
- **TLC Diagram**: The diagram represents a traditional Thin Layer Chromatography (TLC) setup.
- **Key Components**:
- **Solvent Front**: The top line indicating how far the solvent has traveled, marked as 8.4 cm from the baseline.
- **Baseline**: The starting line from which measurements are made.
- **Spots**: Two spots are visible:
- **Bottom Spot**: The distance from the baseline to the bottom spot is 1.4 cm.
- **Top Spot**: Positioned 1.6 cm above the bottom spot.
- **Total Distance Traveled by the Top Spot**: Combining both measurements, the top spot has traveled 1.4 cm + 1.6 cm = 3.0 cm from the baseline.
#### Calculating Rf Value:
- **Formula**: Rf (Retention factor) = Distance traveled by the substance / Distance traveled by the solvent front.
- **Top Spot Rf Calculation**:
- \( \text{Rf} = \frac{3.0 \, \text{cm}}{8.4 \, \text{cm}} = 0.357 \)
The Rf value of the top component is approximately 0.36.
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