13. A student makes a smear by applying a LARGE amount of bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, individual organisms can’t be distinguished. What did the student do wrong when making the smear?
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- 12. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, no organisms are seen. What did the student do wrong when making the smear?14. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, the organisms are misshapen and look weird. What did the student do wrong when making the smear?3. Draw the specimen observed under endospore staining 4. Draw the specimen observed under positive simple staining
- What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handleIn your own words, explain how gram staining is done and how it works.This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordant
- You have a patient sample and have done a Gram Stain. When you look on the microscope, this is what you see. Which of the following tests would you use next? 中 МАС O HE • EMB O MSAWhat is the purpose of fixing a smear? Mark all that apply: To kill the bacteria so they aren't harmed by the staining method O To kill the bacteria to make the slide safer to handle O To attach the bacteria to the slide To break down the cell wall in order to make the cells accept stain To cause the cells to shrink and become distorted Microsoft Bing 11:30 AM do 87 F Sunny 9/14/2021a. After conducting the Gram staining procedures and you observed that all cells are stained with too much purple though the bacteria you fixed is Gram negative, what could be the mistake you have done? b. After conducting the Gram staining procedures and you observed that all cells are stained with too much pink though the bacteria you fixed is Gram positive, what could be the mistake you have done?
- You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purpleYou receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.The first step in identifying an unknown bacterium is to __________. The first step in identifying an unknown bacterium is to __________. culture your unknown on selective media perform endospore staining on your culture prepare a pure working stock simple staining on your culture acid-fast staining on your culture