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- In adoptive immunotherapy for melanoma, tumor-infiltrated lymphocytes(TILs) are expanded ex-vivo then reinfused into the patient together with IL-2. The rationale for the administration of IL-2 is that it serves as a growth factor for the TILs. Group of answer choices True FalseQuestion. If you are working in a diagnostic lab, what will be your choice among Monoclonal or Polyclonal Antibodies method for detection of specific pathogen? Give sufficient justification for selection as well as rejection of any given methods.Discuss the differences between conventional T cell therapy and Chimeric Antigen Receptor (CAR) T cells.
- Question:- describe 3 mechanisms that help generate antibody diversity in naïve B cells and create our naïve BCR repertoire. One of these is the trimming of nucleotides by an exonuclease at the junctions of joined gene segments.Question:- Biology Describe the cellular branch of adaptive immunity and name its key effector cells. Describe how the two types of MHCs present antigens and summarize how MHCs impact transplant rejection. Explain the two-signal mechanism of T cell activation and discuss the factors that affect subclass differentiation2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?
- Describe the significance and need for receptor editing as a part of elimination of self-reactive B lymphocytes.The adaptive response is a robust and critical component of the immune systemis able to combat specific microbial pathogens through the processing and presentation of antigens to specific immune cells Discuss the antigen presentation pathways that are used for viral pathogens and extracellular bacterial pathogens. Include the type of MHC used in each pathwayTrue or false? SDS PAGE gel can be prebed with NBT/BCIP to perform an immunoblot. The direction of proteins through a matrix is due to most proteins in nature having a net negative charge. PBST is buffer that is used for blocking the membrane during immunoloblotting.
- Why can humanized anti-PD-1 is a new powerful immunotherapy for threating cancer?Topic: A patient was diagnosed with lymphoma in 2019. The tumor metastasized to multiple sites of the body. In 2020, the same patient was infected with COVID-19. Upon recovery from the infection, the physician performed a CT scan and noticed tumor complete remission. Using your knowledge in immunology, can you described what may have accounted for the complete remission?In relation to immunotechnology, answer the following: A- Answer the following in relation to Hybridoma technology: → What is the cancer cell line name used in hybridoma technology? → Give an example of murine Monocolonal Antibody produced by hybridoma technology? B- Give ONE main difference between: Murine MABs and fully human MABs? and explain the reason behind developing different forms of Monoclonal antibodies (chimeric MABs, humanized MABs, and fully human MABs) C- You are in an internship in one major Hospital, You were in rotation with a clinical pharmacist in oncology section and you came over a breast cancer patient. The personal doctor was making a quick visit to the patient to make sure the patient is following the therapeutic protocol and the treatment is running smoothly. The doctor wrote in the prescription sheet “Trastuzumab” to be dispensed by the In Patient Pharmacy, when the clinical pharmacist saw this he advised the doctor politely to use the…