11. At what concentration of S (expressed as a multiple of Km) will Vo-0.95 Vmax ? 12. The K", for the reaction of chymotrypsin with N-acetylvaline ethyl ester is 8.8x102M, and the K,. for the reaction of chymotrypsin with N-acetyltyrosine ethyl ester is 6.6x10 4 M. (a) Which substrate has the higher apparent affinity for the enzyme? (b) Which substrate is likely to give a higher value for Vmax? 13. An enzyme catalyzes the reaction A B. The enzyme is present at a concentration of 2 nM, and the Vmax is 1.2 μΜ s-1. The Km for substrate A is 10 μ M. Calculate the initial velocity of the reaction, Vo, when the substrate concentration is (a) 2 μΜ, (b) 10 μΜ, (c) 30 μΜ. 14. An enzyme catalyzes the reaction M N. The enzyme is present at a concentration of 1 nM, and the Vmax is 2 M s-1. The Kn for substrate M is 4 μΜ. Calculate kat.

11. At what concentration of S (expressed as a multiple of Km) will Vo-0.95 Vmax ? 12. The K", for the reaction of chymotrypsin with N-acetylvaline ethyl ester is 8.8x102M, and the K,. for the reaction of chymotrypsin with N-acetyltyrosine ethyl ester is 6.6x10 4 M. (a) Which substrate has the higher apparent affinity for the enzyme? (b) Which substrate is likely to give a higher value for Vmax? 13. An enzyme catalyzes the reaction A B. The enzyme is present at a concentration of 2 nM, and the Vmax is 1.2 μΜ s-1. The Km for substrate A is 10 μ M. Calculate the initial velocity of the reaction, Vo, when the substrate concentration is (a) 2 μΜ, (b) 10 μΜ, (c) 30 μΜ. 14. An enzyme catalyzes the reaction M N. The enzyme is present at a concentration of 1 nM, and the Vmax is 2 M s-1. The Kn for substrate M is 4 μΜ. Calculate kat.

Chemical Principles in the Laboratory

11th Edition

ISBN:9781305264434

Author:Emil Slowinski, Wayne C. Wolsey, Robert Rossi

Publisher:Emil Slowinski, Wayne C. Wolsey, Robert Rossi

Chapter20: Rates Of Chemical Reactions, I. The Iodination Of Acetone

Section: Chapter Questions

Problem 2ASA

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