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10. If an eluent of too high polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate?

10. If an eluent of too high polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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**Educational Content on Thin Layer Chromatography (TLC)** --- **4. Effect of Eluent Polarity on Rf Values** - Did increasing the polarity of your eluent increase or decrease your Rf values? **5. Polarity and Rf Values** - Given that the solid surface is polar, explain why polar samples have lower Rf values than less polar ones. **6. Eluent Polarity and Rf Values** - Explain why increasing eluent polarity increases the Rf value for a given sample. **7. Spot Order for Specific Compounds** - In what order, from top to bottom, would you expect to see the spots for naphthalene (C₁₀H₈), butyric acid (CH₃CH₂CH₂CO₂H), and phenyl acetate (CH₃CO₂C₆H₅)? - **Order**: - Top - Middle - Bottom **8. Spot Order by Compound** - In what order, from top to bottom, would you expect to see the spots for the following? - Acetic acid = CH₃CO₂H - **Top** - Butanal = CH₃CH₂CH₂CHO - **2nd, 3rd, 4th** - 2-octanone = CH₃COCH₂CH₂CH₂CH₂CH₂CH₃ - Decane = C₁₀H₂₂ - **Bottom** - 1-butanol = CH₃CH₂CH₂CH₂OH **9. Eluent of Low Polarity** - If an eluent of too low polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate? **10. Eluent of High Polarity** - If an eluent of too high polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate? --- This content is designed to educate students about the fundamental concepts involved in thin-layer chromatography (TLC), focusing on the role of eluent polarity in determining Rf values and the separation of different compounds.
Transcribed Image Text:**Educational Content on Thin Layer Chromatography (TLC)** --- **4. Effect of Eluent Polarity on Rf Values** - Did increasing the polarity of your eluent increase or decrease your Rf values? **5. Polarity and Rf Values** - Given that the solid surface is polar, explain why polar samples have lower Rf values than less polar ones. **6. Eluent Polarity and Rf Values** - Explain why increasing eluent polarity increases the Rf value for a given sample. **7. Spot Order for Specific Compounds** - In what order, from top to bottom, would you expect to see the spots for naphthalene (C₁₀H₈), butyric acid (CH₃CH₂CH₂CO₂H), and phenyl acetate (CH₃CO₂C₆H₅)? - **Order**: - Top - Middle - Bottom **8. Spot Order by Compound** - In what order, from top to bottom, would you expect to see the spots for the following? - Acetic acid = CH₃CO₂H - **Top** - Butanal = CH₃CH₂CH₂CHO - **2nd, 3rd, 4th** - 2-octanone = CH₃COCH₂CH₂CH₂CH₂CH₂CH₃ - Decane = C₁₀H₂₂ - **Bottom** - 1-butanol = CH₃CH₂CH₂CH₂OH **9. Eluent of Low Polarity** - If an eluent of too low polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate? **10. Eluent of High Polarity** - If an eluent of too high polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate? --- This content is designed to educate students about the fundamental concepts involved in thin-layer chromatography (TLC), focusing on the role of eluent polarity in determining Rf values and the separation of different compounds.
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