10. If an eluent of too high polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate?

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**Educational Content on Thin Layer Chromatography (TLC)**

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**4. Effect of Eluent Polarity on Rf Values**
- Did increasing the polarity of your eluent increase or decrease your Rf values?

**5. Polarity and Rf Values**
- Given that the solid surface is polar, explain why polar samples have lower Rf values than less polar ones.

**6. Eluent Polarity and Rf Values**
- Explain why increasing eluent polarity increases the Rf value for a given sample.

**7. Spot Order for Specific Compounds**
- In what order, from top to bottom, would you expect to see the spots for naphthalene (C₁₀H₈), butyric acid (CH₃CH₂CH₂CO₂H), and phenyl acetate (CH₃CO₂C₆H₅)?
  - **Order**:
    - Top
    - Middle
    - Bottom

**8. Spot Order by Compound**
- In what order, from top to bottom, would you expect to see the spots for the following? 
  - Acetic acid = CH₃CO₂H
    - **Top**
  - Butanal = CH₃CH₂CH₂CHO
    - **2nd, 3rd, 4th**
  - 2-octanone = CH₃COCH₂CH₂CH₂CH₂CH₂CH₃
  - Decane = C₁₀H₂₂
    - **Bottom**
  - 1-butanol = CH₃CH₂CH₂CH₂OH

**9. Eluent of Low Polarity**
- If an eluent of too low polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate?

**10. Eluent of High Polarity**
- If an eluent of too high polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate?

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This content is designed to educate students about the fundamental concepts involved in thin-layer chromatography (TLC), focusing on the role of eluent polarity in determining Rf values and the separation of different compounds.
Transcribed Image Text:**Educational Content on Thin Layer Chromatography (TLC)** --- **4. Effect of Eluent Polarity on Rf Values** - Did increasing the polarity of your eluent increase or decrease your Rf values? **5. Polarity and Rf Values** - Given that the solid surface is polar, explain why polar samples have lower Rf values than less polar ones. **6. Eluent Polarity and Rf Values** - Explain why increasing eluent polarity increases the Rf value for a given sample. **7. Spot Order for Specific Compounds** - In what order, from top to bottom, would you expect to see the spots for naphthalene (C₁₀H₈), butyric acid (CH₃CH₂CH₂CO₂H), and phenyl acetate (CH₃CO₂C₆H₅)? - **Order**: - Top - Middle - Bottom **8. Spot Order by Compound** - In what order, from top to bottom, would you expect to see the spots for the following? - Acetic acid = CH₃CO₂H - **Top** - Butanal = CH₃CH₂CH₂CHO - **2nd, 3rd, 4th** - 2-octanone = CH₃COCH₂CH₂CH₂CH₂CH₂CH₃ - Decane = C₁₀H₂₂ - **Bottom** - 1-butanol = CH₃CH₂CH₂CH₂OH **9. Eluent of Low Polarity** - If an eluent of too low polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate? **10. Eluent of High Polarity** - If an eluent of too high polarity is used for the development of a TLC plate, will the sample spots be somewhere in the middle, way at the bottom, or way at the top of the plate? --- This content is designed to educate students about the fundamental concepts involved in thin-layer chromatography (TLC), focusing on the role of eluent polarity in determining Rf values and the separation of different compounds.
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