1.      Differentiate the following: a.      pH from pOH ____________________________________________________________________________________________________________________________________________________________ b.      Ka from Kb ____________________________________________________________________________________________________________________________________________________________ c.      Pka from Pkb ____________________________________________________________________________________________________________________________________________________________   d.      Kw from pKw

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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1.      Differentiate the following:

a.      pH from pOH

____________________________________________________________________________________________________________________________________________________________

b.      Ka from Kb

____________________________________________________________________________________________________________________________________________________________

c.      Pka from Pkb

____________________________________________________________________________________________________________________________________________________________

 

d.      Kw from pKw

____________________________________________________________________________________________________________________________________________________________

2.      What is the importance of buffer systems in the human body?

________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

3.      What is the Henderson-Hasselbalch equation?

________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

4.      What are the approximate pH ranges and color changes of the following dyes?

 

Indicator

pH range

Color change

Thymol blue (acid range)

 

 

Bromphenol Blue

 

 

Bromcresol Green

 

 

Bromcresol Purple

 

 

Phenol Red

 

 

Thymol Blue (Alkaline range)

 

 

Methyl red – Methylene Blue

 

 

Methyl orange

 

 

Phenolphthalein

 

 

5.      How is the assigned buffer prepared? Write your computations and encircle the amount of solute needed.

 

 

 

 

 

 

 

 

6.      Determination of buffer capacity

Buffer solution

Desired pH

Volume added

Buffer capacity

0.10 M HCl

0.10M NaoH

Acid added

Base added

1

3.0

 

 

 

 

2

4.0

 

 

 

 

3

6.0

 

 

 

 

4

8.0

 

 

 

 

5

9.50

 

 

 

 

6

9.0

 

 

 

 

7

13.0

 

 

 

 

a.      What is the most effective buffer against acids?

____________________________________________________________________________________________________________________________________________________________________________

b.      What is the most effective buffer against bases?

____________________________________________________________________________________________________________________________________________________________________________

c.      What is the most effective buffer against either acids or bases?

____________________________________________________________________________________________________________________________________________________________________________

 

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