1. Differentiate the following: a. pH from pOH ____________________________________________________________________________________________________________________________________________________________ b. Ka from Kb ____________________________________________________________________________________________________________________________________________________________ c. Pka from Pkb ____________________________________________________________________________________________________________________________________________________________ d. Kw from pKw
1. Differentiate the following:
a. pH from pOH
____________________________________________________________________________________________________________________________________________________________
b. Ka from Kb
____________________________________________________________________________________________________________________________________________________________
c. Pka from Pkb
____________________________________________________________________________________________________________________________________________________________
d. Kw from pKw
____________________________________________________________________________________________________________________________________________________________
2. What is the importance of buffer systems in the human body?
________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________
3. What is the Henderson-Hasselbalch equation?
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4. What are the approximate pH ranges and color changes of the following dyes?
Indicator |
pH range |
Color change |
Thymol blue (acid range) |
|
|
Bromphenol Blue |
|
|
Bromcresol Green |
|
|
Bromcresol Purple |
|
|
Phenol Red |
|
|
Thymol Blue (Alkaline range) |
|
|
Methyl red – Methylene Blue |
|
|
Methyl orange |
|
|
Phenolphthalein |
|
|
5. How is the assigned buffer prepared? Write your computations and encircle the amount of solute needed.
6. Determination of buffer capacity
Buffer solution |
Desired pH |
Volume added |
Buffer capacity |
||
0.10 M HCl |
0.10M NaoH |
Acid added |
Base added |
||
1 |
3.0 |
|
|
|
|
2 |
4.0 |
|
|
|
|
3 |
6.0 |
|
|
|
|
4 |
8.0 |
|
|
|
|
5 |
9.50 |
|
|
|
|
6 |
9.0 |
|
|
|
|
7 |
13.0 |
|
|
|
|
a. What is the most effective buffer against acids?
____________________________________________________________________________________________________________________________________________________________________________
b. What is the most effective buffer against bases?
____________________________________________________________________________________________________________________________________________________________________________
c. What is the most effective buffer against either acids or bases?
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