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1. You must prepare 250 ml of (0.1 mM EDTA, 2% w/v SDS, 40 mM NaCl) given 4.68% w/v NaCl (FW 58.44), 0.25 M EDTA, powdered SDS, water. Steps: a. How much 0.25M EDTA will you use? b. How much 4.68% w/v NaCl will you use? c. How much powdered SDS will you use?

1. You must prepare 250 ml of (0.1 mM EDTA, 2% w/v SDS, 40 mM NaCl) given 4.68% w/v NaCl (FW 58.44), 0.25 M EDTA, powdered SDS, water. Steps: a. How much 0.25M EDTA will you use? b. How much 4.68% w/v NaCl will you use? c. How much powdered SDS will you use?

EBK A SMALL SCALE APPROACH TO ORGANIC L
EBK A SMALL SCALE APPROACH TO ORGANIC L
4th Edition
ISBN:9781305446021
Author:Lampman
Publisher:Lampman
Chapter13: Isolation Of Eugenol From Clov
Section: Chapter Questions
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**Preparation Task for Laboratory Solution** **Objective:** Prepare 250 ml of a solution containing the following components: - 0.1 mM EDTA - 2% w/v SDS - 40 mM NaCl **Given Materials:** - 4.68% w/v NaCl (FW 58.44) - 0.25 M EDTA - Powdered SDS - Water **Instructions:** 1. **Calculate the Amounts Needed** **Steps:** a. **EDTA Calculation:** - How much 0.25 M EDTA will you use to achieve a final concentration of 0.1 mM in 250 ml? b. **NaCl Calculation:** - How much 4.68% w/v NaCl will you use to achieve a final concentration of 40 mM in 250 ml? c. **SDS Calculation:** - How much powdered SDS will you use to make a 2% w/v solution in 250 ml? These calculations will help you measure the precise amounts of each component needed to prepare the solution. Make sure to correctly convert units where necessary and use appropriate formulas and molecular weights.
Transcribed Image Text:**Preparation Task for Laboratory Solution** **Objective:** Prepare 250 ml of a solution containing the following components: - 0.1 mM EDTA - 2% w/v SDS - 40 mM NaCl **Given Materials:** - 4.68% w/v NaCl (FW 58.44) - 0.25 M EDTA - Powdered SDS - Water **Instructions:** 1. **Calculate the Amounts Needed** **Steps:** a. **EDTA Calculation:** - How much 0.25 M EDTA will you use to achieve a final concentration of 0.1 mM in 250 ml? b. **NaCl Calculation:** - How much 4.68% w/v NaCl will you use to achieve a final concentration of 40 mM in 250 ml? c. **SDS Calculation:** - How much powdered SDS will you use to make a 2% w/v solution in 250 ml? These calculations will help you measure the precise amounts of each component needed to prepare the solution. Make sure to correctly convert units where necessary and use appropriate formulas and molecular weights.
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