1. You are extracting DNA from 10 mL of whole blood. WBC's (white blood cells) are collected via centrifugation. WBC concentration is 8 x 106 cells / mL, Answer the following questions. a. How many WBC's can you collect from the 10 mL of whole blood? g b. The human genome (haploid) size is 3 x 10° base pairs (bp). The molecular weight of DNA is 660 How much does one copy of human genome weigh? (HINT: How many molecules are there in one mole of a substance?): mol x bp
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
can you help with 1a and 1b please
DNA extraction is a process of isolating DNA (genome) from a given sample of cells. It requires various techniques like alkaline lysis to rupture cells along with purification to isolate intact DNA. Here, the amount of sample given is 10 ml whole blood. This sample contains all kinds of cells including WBC or white blood cells.
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