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- In an in situ hybridization experiment, what is the relationship betweenthe base sequence of the probe DNA and the site on the chromosomal DNA where the probe binds?1.) How are Recombinant DNA formed?What is the difference between genetic modification and selective breeding?What kind of recombination might have the greatest impact onthe core genome?
- 2c) If the whole potoroo genome is 4.2 x 10' bp, and the highlyrepetitive DNA in the potoroo genome is composed entirely ofcopies of the sequence 5'AAGACT' and its complement, howmany copies of this sequence are present in the potoroogenome?what are some Limitations/sources of error with suitable rationalization that may occur during the polymerase chain reaction?Give 2 advantages of SPACER DNA SEQUENCES as regions in the genome/chromosome totarget for DNA marker development.
- The temperature at which the primers and target DNA hybridize may be changed to influence the stringency of PCR amplification. What effect will changing the hybridization temperature have on the amplification? Let's say you have a certain yeast gene A and want to check whether it has a human equivalent. How might managing the hybridization's rigor benefit you?24. The diagram below shows the map for EcoRI restriction sites around the BRCA1 gene for two alleles of the gene, and these alleles. The DNA was digested with the restriction enzyme EcoRI and probed with a radioactively labeled DNA from a cloned copy of the BRCA1 gene. Which individual or individuals are homozygous for allele 1? a southern blot of DNA for four people that are either homozygous for one of the two alleles or are heterozygous fo Southern Blot 12 3 4 Allele 1 Allele 2 BRCA1 EcoRI EcoRI EcoRI 10 kb L A) 1 B) 2 and 3 C) 4 12 kb D) 1 and 4 E) You cannot identify homozygous individuals without data for the offspring of these individuals. ==1. An individual is heterozygous (CT) for a common C/T SNP that is tested using the Axiom microarray (used by the UK Biobank). Two left apex probes at different spots on the microarray will interrogate this SNP, testing both strands of the SNP. Draw the genomic DNA hybridized to both probes and show the dideoxynucleotides added, the fluorescence observed, and the signal recorded at each spot.
- 11. Discuss if you could perform the PCR and then agarose gel electrophoresis using a coding gene instead of a VNTR.12) Draw a yeast knockout cassette. Label all required sequence features. a) Draw the target chromosome if recombination only occurs at one region of homology. b) Draw the target chromosome if recombination occurs using both regions of homology.What is the suitable amounts of genomic DNA to prepare PCR? and what is the suitable purities of the genomic DNA?