1. What is the purpose of preparing a cell culture? 2. Differentiate the general types of cell culture. 3. Enumerate and describe the role of the different components of a cell culture media.
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1. What is the purpose of preparing a cell culture?
2. Differentiate the general types of cell culture.
3. Enumerate and describe the role of the different components of a cell culture media.
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- 1. What are the basic differences from a primary cell culture, cell line and a cell strain? 2. Differentiate the five classes of artificial media used in animal cell culture. 3. What are some of the common problems encountered when performing cell culture?Explain the statements below: 1.Animal cell cultures requires high technical skills to interpret and to regulate animal cell culturing. 2. Animal cell culture makes use of low amounts of reagents.1. Make a timeline of the development and advancement of Microtome. Include the significant inventors and people who contributed in developing the microtome. 2. Illustrate the different types of Microtome. a. Identify its uses b. Identify its different parts and the uses of its parts c. Differentiate how this particular type of microtome being used
- 10. A single cell is placed into a culture tube containing nutrient agar. If the number of cells triples every 2 minutes and the culture tube is completely filled in 12 hours, how long does it take for the culture tube to be only half full of cells?1. Discuss the principle involved with the use of a centrifuge.2. Enumerate some specific examples of application of centrifugation.3. Differentiate the two ways used to express the rate of centrifugation.1a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and high-pH culture conditions. 1b.) Describe the difference between defined and undefined cell culture media. What are the advantages and disadvantages of both of them?
- Explain the statement below: 1. Animal cell culture makes use of low amounts of reagents.1. Determine which technologies are most appropriate for which cell types.TECHNOLOGYCELL TYPE1Plants cells2. Electroporation3. Biolistics4.Bacterial cells5.Mammalian cells1. What is the actual magnification of Cell X? Explain the solution .
- 10. Cell culture methods: culture media and dishes, equipment, culture conditions. Bioreactors.Asap10. Describe the procedure for viable plate count. counting count both living and dead cells? 11. Understand the standard bacterial growth curve and discuss what might be occurring in the culture at each stage. 12. What is flow cytometer? What is use of it? Device used for counting, Sorting, sizing and identifying cells in suspensi'on Chapter 8 1. What is metabolism? How do anabolic and catabolic process differ? Give examples of each. 2. What are enzymes, apoenzymes, holoenzymes, exoenzymes, endoenzymes, constitutive and regulated enzymes, induced and constitutive enzymes? How do they work inside the cell? How are enzyme reactions controlled? 3. Briefly describe how feedback inhibition works. 4. What are competitive and non-competitive inhibitors? 5. List input and output for glycolysis. Where does it occur in pro-karyotic cells and eukaryotic cells? 6. List input and output for the TCA cycle. Where does this cycle occur in the bacterial cell and in eukaryotic cells? 7. Describe the…