1. N 2. Explain the composition of a glycogen molecule Relate the content of liver glycogen to the treatments A, B, C and D.

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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RESULTS:
1.
2.
3.
Plot a graph of [glycogen] (µg/ml) vs A620nm
Calculate glycogen content of A, B, C and D
1.
2.
volume filtrate
livermass
Construct a table showing the following: Treatment and glycogen content (µg/ml)
µg glycogen/g liver = µg/ml (std curve) x
3
QUESTIONS:
Explain the composition of a glycogen molecule
Relate the content of liver glycogen to the treatments A, B, C and D.
DU Anthrone test (in duplicate) as follows. All volumes are in ml.
+ +
+ -1-
4
6
7
0.8
Tube No
Glycogen std (0.1
A620nm
(average)
Blank (1)
0
DESULTA
-
1.6
2
0.2
g/l)
Filtrate
Distilled H₂O
[glycogen] (µg/ml)
Anthrone reagent
4
4
4
4
4
4
Mix well. place in boiling water bath for 10 min and allow to cool in cold water.
A620nm
|||
+
3
0.4
1.4
x dilution factor x
1.2
0.8
65
1.6
1
4
<
8
-
T
A:1.0 B:1.0 C:1.0 D:1.0
0.6
0.6 0.6
0.6
"
(1) 0,07 0,2300335704567 0,9250,656 0,374 9521 0,579
0,052 0,2420,3110 571 0932 0,64 20,3820,5/4/0/589
(2)
4
Transcribed Image Text:RESULTS: 1. 2. 3. Plot a graph of [glycogen] (µg/ml) vs A620nm Calculate glycogen content of A, B, C and D 1. 2. volume filtrate livermass Construct a table showing the following: Treatment and glycogen content (µg/ml) µg glycogen/g liver = µg/ml (std curve) x 3 QUESTIONS: Explain the composition of a glycogen molecule Relate the content of liver glycogen to the treatments A, B, C and D. DU Anthrone test (in duplicate) as follows. All volumes are in ml. + + + -1- 4 6 7 0.8 Tube No Glycogen std (0.1 A620nm (average) Blank (1) 0 DESULTA - 1.6 2 0.2 g/l) Filtrate Distilled H₂O [glycogen] (µg/ml) Anthrone reagent 4 4 4 4 4 4 Mix well. place in boiling water bath for 10 min and allow to cool in cold water. A620nm ||| + 3 0.4 1.4 x dilution factor x 1.2 0.8 65 1.6 1 4 < 8 - T A:1.0 B:1.0 C:1.0 D:1.0 0.6 0.6 0.6 0.6 " (1) 0,07 0,2300335704567 0,9250,656 0,374 9521 0,579 0,052 0,2420,3110 571 0932 0,64 20,3820,5/4/0/589 (2) 4
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