Nutrition: Concepts and Controversies - Standalone book (MindTap Course List)
14th Edition
ISBN:9781305627994
Author:Frances Sizer, Ellie Whitney
Publisher:Frances Sizer, Ellie Whitney
Chapter4: The Carbohydrates: Sugar, Starch, Glycogen, And Fiber
Section: Chapter Questions
Problem 2CT
Related questions
Question
![RESULTS:
1.
2.
3.
Plot a graph of [glycogen] (µg/ml) vs A620nm
Calculate glycogen content of A, B, C and D
1.
2.
volume filtrate
livermass
Construct a table showing the following: Treatment and glycogen content (µg/ml)
µg glycogen/g liver = µg/ml (std curve) x
3
QUESTIONS:
Explain the composition of a glycogen molecule
Relate the content of liver glycogen to the treatments A, B, C and D.
DU Anthrone test (in duplicate) as follows. All volumes are in ml.
+ +
+ -1-
4
6
7
0.8
Tube No
Glycogen std (0.1
A620nm
(average)
Blank (1)
0
DESULTA
-
1.6
2
0.2
g/l)
Filtrate
Distilled H₂O
[glycogen] (µg/ml)
Anthrone reagent
4
4
4
4
4
4
Mix well. place in boiling water bath for 10 min and allow to cool in cold water.
A620nm
|||
+
3
0.4
1.4
x dilution factor x
1.2
0.8
65
1.6
1
4
<
8
-
T
A:1.0 B:1.0 C:1.0 D:1.0
0.6
0.6 0.6
0.6
"
(1) 0,07 0,2300335704567 0,9250,656 0,374 9521 0,579
0,052 0,2420,3110 571 0932 0,64 20,3820,5/4/0/589
(2)
4](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F55f2e864-1b25-4c19-a3d8-9c2af89a719d%2F50f2c359-e9b7-48b6-858d-d215013d7102%2Ff0hch06f_processed.jpeg&w=3840&q=75)
Transcribed Image Text:RESULTS:
1.
2.
3.
Plot a graph of [glycogen] (µg/ml) vs A620nm
Calculate glycogen content of A, B, C and D
1.
2.
volume filtrate
livermass
Construct a table showing the following: Treatment and glycogen content (µg/ml)
µg glycogen/g liver = µg/ml (std curve) x
3
QUESTIONS:
Explain the composition of a glycogen molecule
Relate the content of liver glycogen to the treatments A, B, C and D.
DU Anthrone test (in duplicate) as follows. All volumes are in ml.
+ +
+ -1-
4
6
7
0.8
Tube No
Glycogen std (0.1
A620nm
(average)
Blank (1)
0
DESULTA
-
1.6
2
0.2
g/l)
Filtrate
Distilled H₂O
[glycogen] (µg/ml)
Anthrone reagent
4
4
4
4
4
4
Mix well. place in boiling water bath for 10 min and allow to cool in cold water.
A620nm
|||
+
3
0.4
1.4
x dilution factor x
1.2
0.8
65
1.6
1
4
<
8
-
T
A:1.0 B:1.0 C:1.0 D:1.0
0.6
0.6 0.6
0.6
"
(1) 0,07 0,2300335704567 0,9250,656 0,374 9521 0,579
0,052 0,2420,3110 571 0932 0,64 20,3820,5/4/0/589
(2)
4
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