A baby fails to thrive after 8 weeks of life. She wakes frequently and is very irritable.The parents notice that the baby appears to be better after feeding but that herabdomen is protruding more and more. After her condition worsens, she is admittedto hospital where an examination indicates that his liver and kidneys areenlarged. A liver biopsy was performed and a liver extract was prepared byhomogenising 200mg of liver in 1ml of 0.3M sucrose, 10mM Tris-HCl, pH 7.5, 1mMdithiothreitol, 1mM EGTA and 100μM phenylmethylsulphonyl fluoride (PMSF). Theactivity of enzyme X was measured in the liver sample by adding 100μl of the liverextract to 0.9 ml of 120mM Imidazole buffer (pH 7.0) containing 2.4mM NAD+*, 30mMglucose-6-phosphate, 6 units/ml of glucose dehydrogenase in a cuvette with a 1cmlight path. The increase in absorbance at 340nm was followed over a period of 10minutes and compared with a blank sample containing everything but the glucose-6-phosphate. The results are shown in the table below:Time(mins)0246810Absorbance at 340nmWith G6P00.120.230.340.440.53Without G6P00.030.0620.0820.0870.0921. From the constituents of the assay, identify "enzyme X" and write out thereaction it catalyses, and the coupling reaction. State why the coupling reactionis needed.

Glucose dehydrogenase (GDH) is oxidoreductases enzyme which catalyzes the conversion of glucose to…

Answered: 1. From the constituents of the assay,…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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What does it mean to hydrolyze RNA before doing biochemical tests?
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Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…
a.) Explain briefly how silica allows us to purify DNA. Be sure to state the relevant biochemical features of silica and DNA as well as any relevant reagents or steps that are important for the purification. Your answer should explain both how we get the DNA to bind in the first place, and how we are able to remove the DNA. You do not (and should not) explain earlier steps in a DNA purification; focus you answer on the silica:DNA interaction. b.) In theory, can a silica spin column also be used to purify RNA? If so, explain how (and why we did not observe RNA when analyzing plasmid DNA via agarose gel electrophoresis). If not, explain why not.
Consider the following restriction enzymes, which cut at the positions indicated by arrows: Which enzymes produce ends that are compatible with those of enzyme E? Select all that apply. Enzyme H Enzyme F Enzyme G
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1. From the constituents of the assay, identify "enzyme X" and write out the reaction it catalyses, and the coupling reaction. State why the coupling reaction is needed.