A new drug was developed and found to be primarily eliminated by hepaticmetabolism; i.e., the drug was not excreted in urine or secreted into bile.metakof the metabolic reaction is 2 mg/L and its Vmax is 125 mg/d. Usingthe Michaelis-Menten equation, calculate the rate of metabolism when theplasma drug concentration [C,] is as shown in the table below.[C,] mg/LRate of Metabolism (mg/d)0.0250.0500.100.200.51.02.04.010204060Plot a graph with concentration on the X-axis and rate of metabolismon the Y-axis. Note how the reaction rate increases proportionately withplasma concentration at low concentrations (first-order kinetics), and thenapproaches saturation (Vmax) at high concentrations.nictored orally as a 159 mg tablet. During

According to Michaelis-Menten Kinetics, when the rate of metabolism or velocity of an enzyme catalyzed reaction is measured at varying substrate concentrations, the rate depends on the substrate concentrations [S]. At a relatively low concentration of substrate, initial velocity (V) or metabolism rate increases almost linearly with an increase in substrate concentration. The reaction reaches a maximum velocity (Vmax) with an increase in substrate concentration and it does not increase any further by increasing in substrate concentration. Concentration of substrate at which the reaction velocity reaches half of the maximum velocity is called Michaelis constant or Km. Michaelis and Menten have derived a mathematical equation to establish the relationship among the concentration of enzyme, substrate, Vmax, Km as follows: Here, Km = 2 mg/L Vmax = 125 mg/d Lets assume rate of metabolism is defined by V and substrate concentration is denoted by S.