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1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µl as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 pl of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of DH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µl 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram

1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µl as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 pl of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of DH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µl 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram

Chemistry & Chemical Reactivity
Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter11: Intermolecular Forces And Liquids
Section11.6: Properties Of Liquids
Problem 2.2ACP
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1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µL as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 µL of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of dH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram
Transcribed Image Text:1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µL as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 µL of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of dH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram
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