Homework Help is Here – Start Your Trial Now!
Science
Chemistry
1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µl as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 pl of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of DH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µl 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram

1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µl as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 pl of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of DH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µl 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
See similar textbooks
icon
Related questions
Question
1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µL as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 µL of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of dH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram
Transcribed Image Text:1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µL as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 µL of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of dH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 5 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Measurement
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
SEE MORE TEXTBOOKS