1) Determine the total volume of the standards by using a formula in the appropriate boxes (your instructor will help you with this). 2) Determine the concentration of Cu2+ in each standard, also using a formula in the appropriate cells. 3) Plot the absorbance as a function of Cu2+ concentration. Determine the equation for the best-fit line.

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Can I please get help on steps 1-3

 

Directions:
(1) Determine the total volume of the standards by using a formula in the appropriate boxes (your instructor will help you with this).
(2) Determine the concentration of Cu2+ in each standard, also using a formula in the appropriate cells.
(3) Plot the absorbance as a function of Cu2+ concentration. Determine the equation for the best-fit line.
1.0 M ethylenediamine
(mL)
concentration Cu2+ absorbance (AU)
at 550 nm
0.01 M CuSO4 5H2O
H2O
total volume
stock Cu2+
standard
(mL)
(mL)
(mL)
(units)
concentration (M)
1
0.50
0.00
9.50
0.025
0.01
2
0.50
0.50
9.00
0.031
0.01
3
0.50
1.00
8.50
0.048
0.01
4
0.50
2.00
7.50
0.153
0.01
5
0.50
3.00
6.50
0.223
0.01
0.50
4.00
5.50
0.307
0.01
7
0.50
5.00
4.50
0.384
0.01
Transcribed Image Text:Directions: (1) Determine the total volume of the standards by using a formula in the appropriate boxes (your instructor will help you with this). (2) Determine the concentration of Cu2+ in each standard, also using a formula in the appropriate cells. (3) Plot the absorbance as a function of Cu2+ concentration. Determine the equation for the best-fit line. 1.0 M ethylenediamine (mL) concentration Cu2+ absorbance (AU) at 550 nm 0.01 M CuSO4 5H2O H2O total volume stock Cu2+ standard (mL) (mL) (mL) (units) concentration (M) 1 0.50 0.00 9.50 0.025 0.01 2 0.50 0.50 9.00 0.031 0.01 3 0.50 1.00 8.50 0.048 0.01 4 0.50 2.00 7.50 0.153 0.01 5 0.50 3.00 6.50 0.223 0.01 0.50 4.00 5.50 0.307 0.01 7 0.50 5.00 4.50 0.384 0.01
(4) Using your calibration curve, determine the concentration of Cu2+ in unknown A.
(5) Next, calculate the concentration of Cu2+ in the stock solution A used to make unknown A.
volume of CUSO4
absorbance (AU)
1.0 M ethylenediamine
(mL)
H2O
total volume
concentration Cu2+
stock A Cu2+
unknown
5H20 of unknown
(mL)
(mL)
(units)
at 550 nm
concentration (M)
conc; stock A (mL)
A
0.50
2.00
7.50
0.25
(6) Repeat steps 4-5 for unknowns B and C.
volume of CuSO4
1.0 M ethylenediamine
(mL)
total volume
(mL)
concentration Cu2+ absorbance (AU)
at 550 nm
H2O
stock B Cu2+
unknown
5H2O of unknown
(mL)
(units)
concentration (M)
conc; stock B (mL)
В
0.50
3.00
6.50
0.451
volume of CuSO4
concentration Cu2+| absorbance (AU)
1.0 M ethylenediamine
(mL)
H2O
total volume
stock C Cu2+
unknown
5H2O of unknown
(mL)
(mL)
(units)
at xx nm
concentration (M)
conc; stock C (mL)
0.50
4.50
5.00
0.283
Transcribed Image Text:(4) Using your calibration curve, determine the concentration of Cu2+ in unknown A. (5) Next, calculate the concentration of Cu2+ in the stock solution A used to make unknown A. volume of CUSO4 absorbance (AU) 1.0 M ethylenediamine (mL) H2O total volume concentration Cu2+ stock A Cu2+ unknown 5H20 of unknown (mL) (mL) (units) at 550 nm concentration (M) conc; stock A (mL) A 0.50 2.00 7.50 0.25 (6) Repeat steps 4-5 for unknowns B and C. volume of CuSO4 1.0 M ethylenediamine (mL) total volume (mL) concentration Cu2+ absorbance (AU) at 550 nm H2O stock B Cu2+ unknown 5H2O of unknown (mL) (units) concentration (M) conc; stock B (mL) В 0.50 3.00 6.50 0.451 volume of CuSO4 concentration Cu2+| absorbance (AU) 1.0 M ethylenediamine (mL) H2O total volume stock C Cu2+ unknown 5H2O of unknown (mL) (mL) (units) at xx nm concentration (M) conc; stock C (mL) 0.50 4.50 5.00 0.283
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